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1、猪的肠道疾病猪的肠道疾病-尸体解剖检查步骤尸体解剖检查步骤Enteric Diseases of Swine-Postmortem Techniques Kent J.Schwartz,DVM MS 1999 Pharmacia&Upjohn Company1死猪是容易得到的，剖检死猪也可得到有用的资料Dead pigs are most available and may yield useful information,however慢性病猪的原发病原可能会被继发病原所掩盖Primary causes of disease may be overshadowed by secondary
2、agents in pigs that have chronic disease 病死猪A pig that died because of disease尸体解剖技术尸体解剖技术Postmortem Techniques 准备工作准备工作Preparing for the Examination2尸体剖检技术尸体剖检技术Postmortem Techniques 准备工作准备工作Preparing for the Examination理想的是屠宰急性病猪，以检查主要病原It is desirable to euthanize acutely affected pigs to detect
3、primary pathogens 通常剖检多个尸体，以确定主要病变是一致的Usually multiple carcasses are opened until it is clear that the predominant lesions are consistently present in all pigs 刚刚屠宰的病猪Freshly euthanized pig3将猪腹面朝上Place the pig lying on its back,然后从两侧腋下切至颌骨Incise from axilla to between jawbones on both sides 尸体剖检步骤Be
4、gin postmortem procedure尸体剖检技术尸体剖检技术Postmortem Techniques 准备工作准备工作Preparing for the Examination4从两侧腹股沟切至会阴Incise from inguinal region to perineum on both sides,在髋臼处分离两侧股骨Free femur from the acetabulum on both sides.分离髋关节Spreading the hip joints尸体剖检步骤尸体剖检步骤Postmortem Techniques 准备工作准备工作Preparing for
5、the Examination5将猪腹部朝上With the pig lying on its back,分开四肢All four limbs are detached and the carcass will not roll over 分开四肢All limbs are detached尸体剖检工作尸体剖检工作Postmortem Techniques 准备工作准备工作Preparing for the Examination6 抓住皮肤Creating a“handle”抓住颌骨下方皮肤Make a handle from the skin under the chin,and 从胸骨下
6、穿过肋软骨关节切开Incise below breastbone through costochondral junctions尸体剖检工作尸体剖检工作Postmortem Techniques 准备工作准备工作Preparing for the Examination7 打开胸腔（C）Opening the thoracic cavity(C)小心打开胸腔Open the thoracic cavity carefully 观察心、肺周围粘连及体液渗出情况Observe for signs of adhesions and excess fluid around the heart and
7、lungs尸体剖检技术尸体剖检技术Postmortem Techniques 准备工作准备工作Preparing for the Examination8C 打开胸腔Opening the abdominal cavity继续切至尾部，打开胸腔Continue the incision caudally,opening the abdominal cavity尸体剖检技术尸体剖检技术Postmortem Techniques 准备工作准备工作Preparing for the Examination9 分离头两条肋骨Separating the first two ribs从腹中线切开头两条肋
8、骨Cut between the first two ribs,on the mid-line尸体剖检技术尸体剖检技术Postmortem Techniques 准备工作准备工作Preparing for the Examination10 打开胸腔Opening the chest cavity用手将胸腔扩张开Manually spread the chest cavity open检查浆膜表面粘连和体液渗出情况Inspect the serosal surfaces for evidence of adhesions or excess fluids尸体剖检技术尸体剖检技术Postmort
9、em Techniques 准备工作准备工作Preparing for the Examination11尸体剖检技术尸体剖检技术Postmortem Techniques 呼吸道的检查呼吸道的检查Examination of the Respiratory Tract 扁桃体（T）的检查Examination of the tonsils(T)在呼吸道或部分呼吸道被切除前，检查扁桃体The tonsils can be examined before the respiratory tract is removed,or as part of the intact tract 观察扁桃体及气
10、管炎症Look for inflammation of the tonsils and trachea12T尸体剖检技术尸体剖检技术Postmortem Techniques 呼吸道的检查呼吸道的检查Examination of the Respiratory Tract13 切下全部的呼吸道The respiratory tract is removed intact从“干净”的器官开始Begin with the“clean”organs first将整个呼吸道及舌、扁桃体一起切开Remove the entire respiratory tract intact,with tongue
11、and tonsils attached心脏Heart肺Lungs14尸体剖检技术尸体剖检技术Postmortem Techniques 心和肺的检查心和肺的检查Examination of the Heart and Lungs 心和肺的检查Examination of the heart and lungs打开心房，检查瓣膜Open heart chambers,examine the valves按压肺脏，以检查硬度和密度Palpate the lung for firmness and consistency沿气管切开肺脏，检查肺部实质Slice into the lung,acros
12、s airways,looking for areas of consolidation15尸体剖检技术尸体剖检技术Postmortem Techniques 腹腔器官的检查腹腔器官的检查Examination of the Abdominal Organs 肾脏的检查Examination of the kidney检查切开并取出肾脏和膀胱Examine,cut,and remove the kidneys and urinary bladder观察出血或脓肿Look for signs of hemorrhage or abscesses肾肾Kidney16尸体剖检步骤尸体剖检步骤Post
13、mortem Techniques 腹腔器官的检查腹腔器官的检查Examination of the Abdominal Organs 脾脏的检查Examination of the spleen检查脾脏，如果要采集病料，切除脾脏Examine spleen,cut and remove if specimens are to be collected观察脾表面肿大或出血情况Look for signs of enlargement or hemorrhage on the surface脾脾Spleen17尸体剖检技术尸体剖检技术Postmortem Techniques 腹腔器官的检查腹腔
14、器官的检查Examination of the Abdominal Organs 肝脏的检查Examination of the liver检查肝脏，如果要采集病料，切除肝脏Examine the liver,cut and remove if specimens are to be collected观察肝脏表面肿大或出血情况Look for signs of enlargement or hemorrhage on the surfaces of this important organ 肝脏肝脏Liver胆囊胆囊Gall bladder18尸体剖检技术尸体剖检技术Postmortem T
15、echniques 腹腔器官的检查腹腔器官的检查Examination of the Abdominal Organs回肠（I）盲肠（C）和肠系膜淋巴结（L）的检查Examination of the ileum(I),cecum(C)and mesentericlymph nodes(L)检查回肠和盲肠的外表面Examine external surfaces of the ileum and cecum观察肠系膜淋巴结肿胀情况Look for signs of enlargement of the mesenteric lymph nodes切除整个消化道Remove the entire
16、 digestive tractCLI结肠结肠Colon19尸体剖检技术尸体剖检技术Postmortem Techniques 腹腔器官的检查腹腔器官的检查Examination of the Abdominal Organs结肠袢的检查Examination of the spiral colon检查结肠外表面Examine the external surfaces of the colon,then open the lumen打开肠腔，观察炎症情况Open the lumen,look for signs of inflammation观察肠系膜淋巴结肿大情况Look for sign
17、s of enlargement of the mesenteric lymph nodes20尸体剖检技术尸体剖检技术Postmortem Techniques 腹腔器官的检查腹腔器官的检查Examination of the Abdominal Organs检查胃壁Examination of the stomach lining检查胃的外表面Examine the external surfaces of the stomach切开胃，观察炎症和溃疡Open the stomach,look for signs of inflammation and ulceration胃胃Stomac
18、h21尸体剖检技术尸体剖检技术Postmortem Techniques 脑的检查脑的检查Examination of the Brain在第一颈椎处切断脊髓，取下头部（V）Removing the head by severingthe spinal cord at thefirst vertebrae(V)在头和第一椎骨之间切断脊骨，取下头部Remove the head by severing the spinal cord between the head and first vertebraV22尸体剖检技术尸体剖检技术Postmortem Techniques 脑的检查脑的检查Ex
19、amination of the Brain准备取出脑Preparing to remove the brain打开颅骨Open the skull如果猪体重10公斤，纵向劈开头骨Split the head on the midline(lengthwise)in pigs 10公斤,用斧子或切肉刀在眼睛后侧横向劈开In pigs 10 kg,use a hatchet or cleaver to chop transversely behind the eyes23尸体剖检技术尸体剖检技术Postmortem Techniques 脑的检查脑的检查Examination of the Br
20、ain从矢状面切开Chopping in a sagital direction首先,从眼睛后侧横向切开First,chop transversely behind the eyes然后,沿脊髓径直从矢状面切开,暴露脑Next,chop in sagital direction from the level of the spinal cord to expose the brain24尸体剖检技术尸体剖检技术Postmortem Techniques 脑的检查脑的检查Examination of the Brain准备取出脑The brain is ready to be removed去除
21、颅骨暴露出脑Pull back the part of the skull that has been loosened to expose the brain取出部分脑,放入福尔马林溶液,剩余部分冷冻,用作组织病理学和微生物学检查Place part of the brain in formalin solution,chill the remainder for histopathological and microbiological examination25尸体剖检技术尸体剖检技术Postmortem Techniques 鼻甲骨的检查鼻甲骨的检查Examination of the
22、 Nasal Turbinates找出第二前臼齿(P)Locate the second premolar(P)检查鼻甲骨,在第二前臼齿水平横向锯开口鼻部To examine the turbinates,saw the snout transversely at the level of the second premolar toothP26尸体剖检技术尸体剖检技术Postmortem Techniques 鼻甲骨的检查鼻甲骨的检查Examination of the Nasal Turbinates暴露出鼻甲骨(P)Nasal turbinates exposed(T)鼻甲骨萎缩是萎缩性
23、鼻炎的症状The presence of turbinate atrophy is a sign of atrophic rhinitis用标准图表来评定鼻甲骨萎缩的程度Score the degree of turbinate atrophy using a standard rating chartT27尸体剖检技术尸体剖检技术Postmortem Techniques 鉴别诊断肠炎的简单实验鉴别诊断肠炎的简单实验Simple Tests to Differentiate EnteritisPH试纸可以鉴别诊断仔猪下痢的原因pH paper can differentiate thecau
24、se of diarrhea in piglets粪便的PH值可以反映仔猪下痢的原因Fecal(cecal)pH may reveal the cause of diarrhea in pigletsPH7.0,说明分泌过多,可能是由大肠杆菌引起pH7.0-indicates hypersecretion,likely caused by E.coliPH7.0,说明吸收障碍,说明可能是病毒或球虫感染引起pH7.0-indicates malabsorption,likely caused by viral or coccidia infection28尸体剖检技术尸体剖检技术Postmort
25、em Techniques 鉴别诊断肠炎的简单试纸鉴别诊断肠炎的简单试纸Simple Tests to Differentiate Enteritis压印片Preparing an impression smear小肠压印片可以查出未成熟的球虫Impression smears from the small intestine may demonstrate immature coccida用粘膜制备压印片,干燥,然后用Diff-Quick或瑞特染色Press slide again mucosa,air dry and stain with Diff-Quick or Wrights sta
26、in检查裂殖子Examine for merozoites29尸体剖检技术尸体剖检技术Postmortem Techniques 鉴别诊断肠炎的简单实验鉴别诊断肠炎的简单实验Simple Tests to Differentiate Enteritis怀疑是猪痢疾时,从结肠刮取物可以查出是否有螺旋体Colon scrapings may demonstratespirochaets in suspected cases ofswine dysentery刮取病变结肠黏膜Scrape affected colon mucosa用载波片制备压印片Make thin smear on slide用维多利亚蓝或结晶紫染色20分钟Stain with Victoria Blue or Crystal Violet for 20 minutes在400倍显微镜下检查螺旋体Examine at 400X for spiral-shaped organisms30Thank You!