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1、Genetic engineeringGenetic engineeringPARTChapter II02Section Six Whole Genome Amplification and Genomic Library(1).DNA template:To be replicated DNA helicase(2).Primer：DNA polymerase extends on it to synthesize progeny DNA(3).DNA polymerase：DNA synthase(4).DNA Synthase reaction buffer：Provides ion
2、conditions required for enzyme reaction,etc.(5).ATP、CTP、GTP、TTP：four deoxynucleotides required for DNA synthesis.Whole-genome amplification(WGA)based on multiple displacement amplification(MDA)Phi29 DNA polymerase1.Continuous synthesis ability:70 kb2.No requirements for Template DNA:Single-stranded
3、or double-stranded will do,and circular or linear will do.3.Fast amplification speed,50nt/s4.Large synthetic product,3-5 micrograms5.Corrective activity6.The reaction temperature is 30 degrees Celsius.Genomic libraryA library of DNA fragments containing the entire sequence of the entire genome1.Prep
4、arations of DNA fragments2.Construction vector3.Transfer into recipient cellsThe mystery of life lies in the“Four-character Gospel Truth”The Human Genome ProjectFirst Chief Scientist：James WatsonSecond Chief Scientist：Francis CollinsShotgun sequencinghttps:/gold.jgi.doe.gov/indexTHANK YOUThanks for listening