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1、会计学1生物生物(shngw)反应器反应器Newtechnologiesincellculture第一页，共33页。Contentn nIntroductionn nComparisonn nSensor technologies第1页/共34页第二页，共33页。Introduction-bioreactors第2页/共34页第三页，共33页。Introduction-bioreactors Capable of being operated aseptically for a number of Capable of being operated aseptically for a numb
2、er of days,weeks days,weeks and even monthsand even months Capable of generating Capable of generating repeatablerepeatable process conditions for a number of days,weeks and even process conditions for a number of days,weeks and even monthsmonths Adequate Adequate aeration aeration andand agitation
3、agitation should be provided to meet the metabolic requirements should be provided to meet the metabolic requirements of the micro-organism or cellof the micro-organism or cell pH,Oxygen,TemperaturepH,Oxygen,Temperature and other control should be provided and other control should be provided Sampli
4、ngSampling facilities should be provided facilities should be provided Should be suitable for a range of processesShould be suitable for a range of processes Should be constructed to ensure smooth internal surfaces(cleanability)Should be constructed to ensure smooth internal surfaces(cleanability)第3
5、页/共34页第四页，共33页。-Flask第4页/共34页第五页，共33页。n nTest Tubes,Shake flasks.Test Tubes,Shake flasks.n nIncreased aeration.Increased aeration.n nReduced evaporative loss.Reduced evaporative loss.n nSolid support,Re-useable/Autoclavable.Solid support,Re-useable/Autoclavable.n nMay be used with the SensorDish Rea
6、derMay be used with the SensorDish Reader.-Flasks are Shake Flasks第5页/共34页第六页，共33页。-Flask Configurations-Flask ConfigurationsFrom 100ul to 4.0mlFrom 100ul to 4.0ml第6页/共34页第七页，共33页。SensorDish Reader,SDR第7页/共34页第八页，共33页。SensorDish Reader,SDR1.Non-invasive,online.2.pH or Dissolved Oxygen.3.No Calibrati
7、on Required.6-well24-well第8页/共34页第九页，共33页。ExpandableAdditional SDRs link into the Basic Set.Up to 10 SDRs on one Laptop.No additional software required for expansion.第9页/共34页第十页，共33页。SDRs Integrate with -Flasks-Flasks Integrate with the SensorDishReader1.Eliminate Well-plate inconsistency.2.Reduce a
8、nd equalize evaporative losses across well-plates.3.Obtain online pH or dissolved oxygen data for all wells.第10页/共34页第十一页，共33页。DO profiles(all 24 for each well-plate)(all 24 for each well-plate)Polystyreneu-Flask第11页/共34页第十二页，共33页。SensorFlaskReader,SFR第12页/共34页第十三页，共33页。The SensorFlask Reader,SFRSFR
9、 Shaker-mounting blue-tooth sensor platform.Shaker-mounting blue-tooth sensor platform.Shake Flasks with DO Shake Flasks with DO andand pH sensors.pH sensors.(also single DO or pH if desired)(also single DO or pH if desired)Multiple volumes(125/250/500/1000 ml).Multiple volumes(125/250/500/1000 ml).
10、Up to 9 flasks on the platform at one time.Up to 9 flasks on the platform at one time.第13页/共34页第十四页，共33页。Microbial and Mammalian Applicationsn nMicrobial Applications:Microbial Applications:Monitor pH and DO levels.Monitor pH and DO levels.Ensure aerobic growth.Ensure aerobic growth.Follow carbon so
11、urce utilization and exhaustion.Follow carbon source utilization and exhaustion.Adjust orbiter speeds to maintain DO levels for Adjust orbiter speeds to maintain DO levels for better scalability.better scalability.n nMammalian Applications:Mammalian Applications:Accurately monitor pH/pCO2 levels.Acc
12、urately monitor pH/pCO2 levels.Adjust bicarbonate levels more accurately.Adjust bicarbonate levels more accurately.Monitor DO levels,adjust orbiter speeds for better scalability.Monitor DO levels,adjust orbiter speeds for better scalability.第14页/共34页第十五页，共33页。Software第15页/共34页第十六页，共33页。Individual Fl
13、ask Calibration第16页/共34页第十七页，共33页。Complete HardwareComplete Hardware Hardware Includes:Hardware Includes:Platform.Platform.Batteries.Batteries.Bluetooth adapter.Bluetooth adapter.Software.Software.Clamps.Clamps.Bluetooth range of 15ft.Bluetooth range of 15ft.Battery life 3 months on a single charge.
14、Battery life 3 months on a single charge.(platform(platform can operate on single battery)can operate on single battery)第17页/共34页第十八页，共33页。The RAMBIO1000(Resonant Acoustic Mixing)第18页/共34页第十九页，共33页。Resonant Acoustic MixingResonant Acoustic MixingAll energy goes into mixing the contents of the flask6
15、 fold better OTRs than orbital shakers due to increased headspace exchangeUp to 50%fill volume in standard flasks第19页/共34页第二十页，共33页。The Applikon RAMAccessories:Clamps and Oxy-pump Stoppers250ml1000ml第20页/共34页第二十一页，共33页。Single use applicationsn nSmall scale options,more R&DAppliFlex CellReady第21页/共34
16、页第二十二页，共33页。Sensor technologiesn nTraditional sensor pH and DOn nSterilized via autoclave第22页/共34页第二十三页，共33页。Sensor technologies-Fluorescentn nFluorescent light is measure for pH and DO第23页/共34页第二十四页，共33页。Fluorescence pro/conn nPre-sterilizedn nPre-calibrated(pH)n nSingle use+reusable(DO)n nMeasurem
17、ent through wall of vesseln npH range limited n nNot backwards compatible(controller)n nCan be influenced by medium(pH)n nAdditional costPROCON第24页/共34页第二十五页，共33页。New technologiesn nAppliFlex reusableAppliFlex single sensorsuse sensor第25页/共34页第二十六页，共33页。New technologiesn nReusable standardn nSingle
18、sensors use Sensor optionalEz-ControlEz-Control第26页/共34页第二十七页，共33页。Small scale bioreactors第27页/共34页第二十八页，共33页。Small scale bioreactor system29第28页/共34页第二十九页，共33页。Small scale bioreactorn nCommunication via web browserCommunication via web browsern nSoftware to do calibration/setpoints/control loops on Software to do calibration/setpoints/control loops on multiple systems simultaneouslymultiple systems simultaneouslyn nParallel cultivations?Parallel cultivations?第29页/共34页第三十页，共33页。BioSepAcoustic cell separation第30页/共34页第三十一页，共33页。BioSep第31页/共34页第三十二页，共33页。BioSep第32页/共34页第三十三页，共33页。