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1、ContentIntroductionComparisonSensor technologies第1页/共34页Introduction-bioreactors第2页/共34页Introduction-bioreactorsCapable of being operated aseptically for a number of days,weeks and even monthsCapable of generating repeatable process conditions for a number of days,weeks and even monthsAdequate aerat
2、ion and agitation should be provided to meet the metabolic requirements of the micro-organism or cellpH,Oxygen,Temperature and other control should be providedSampling facilities should be providedShould be suitable for a range of processesShould be constructed to ensure smooth internal surfaces(cle
3、anability)第3页/共34页-Flask第4页/共34页Test Tubes,Shake flasks.Increased aeration.Reduced evaporative loss.Solid support,Re-useable/Autoclavable.May be used with the SensorDish Reader.-Flasks are Shake Flasks第5页/共34页-Flask ConfigurationsFrom 100ul to 4.0ml第6页/共34页SensorDish Reader,SDR第7页/共34页SensorDish Rea
4、der,SDR1.Non-invasive,online.2.pH or Dissolved Oxygen.3.No Calibration Required.6-well24-well第8页/共34页ExpandableAdditional SDRs link into the Basic Set.Up to 10 SDRs on one Laptop.No additional software required for expansion.第9页/共34页SDRs Integrate with -Flasks-Flasks Integrate with the SensorDishRea
5、der1.Eliminate Well-plate inconsistency.2.Reduce and equalize evaporative losses across well-plates.3.Obtain online pH or dissolved oxygen data for all wells.第10页/共34页DO profiles(all 24 for each well-plate)Polystyreneu-Flask第11页/共34页SensorFlaskReader,SFR第12页/共34页The SensorFlask Reader,SFRShaker-moun
6、ting blue-tooth sensor platform.Shake Flasks with DO and pH sensors.(also single DO or pH if desired)Multiple volumes(125/250/500/1000 ml).Up to 9 flasks on the platform at one time.第13页/共34页Microbial and Mammalian ApplicationsMicrobial Applications:Monitor pH and DO levels.Ensure aerobic growth.Fol
7、low carbon source utilization and exhaustion.Adjust orbiter speeds to maintain DO levels for better scalability.Mammalian Applications:Accurately monitor pH/pCO2 levels.Adjust bicarbonate levels more accurately.Monitor DO levels,adjust orbiter speeds for better scalability.第14页/共34页Software第15页/共34页
8、Individual Flask Calibration第16页/共34页Complete HardwareHardware Includes:Platform.Batteries.Bluetooth adapter.Software.Clamps.Bluetooth range of 15ft.Battery life 3 months on a single charge.(platform can operate on single battery)第17页/共34页The RAMBIO1000(Resonant Acoustic Mixing)第18页/共34页Resonant Aco
9、ustic MixingAll energy goes into mixing the contents of the flask6 fold better OTRs than orbital shakers due to increased headspace exchangeUp to 50%fill volume in standard flasks第19页/共34页The Applikon RAMAccessories:Clamps and Oxy-pump Stoppers250ml1000ml第20页/共34页Single use applicationsSmall scale o
10、ptions,more R&DAppliFlex CellReady第21页/共34页Sensor technologiesTraditional sensor pH and DOSterilized via autoclave第22页/共34页Sensor technologies-FluorescentFluorescent light is measure for pH and DO第23页/共34页Fluorescence pro/conPre-sterilizedPre-calibrated(pH)Single use+reusable(DO)Measurement through
11、wall of vesselpH range limited Not backwards compatible(controller)Can be influenced by medium(pH)Additional costPROCON第24页/共34页New technologiesAppliFlex reusableAppliFlex single sensorsuse sensor第25页/共34页New technologiesReusable standardSingle sensors use Sensor optionalEz-Control第26页/共34页Small sca
12、le bioreactors第27页/共34页Small scale bioreactor system28第28页/共34页Small scale bioreactorCommunication via web browserSoftware to do calibration/setpoints/control loops on multiple systems simultaneouslyParallel cultivations?第29页/共34页BioSepAcoustic cell separation第30页/共34页BioSep第31页/共34页BioSep第32页/共34页Questions?第33页/共34页