与DRE元件结合的转录因子的克隆和结构分析.pdf

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31、#6$-)+,#-$#*$-,.&4 M8=-,&2.9AH 0.!$,.?93.$-&/.+,&3+&,!-)!./0!$!$.*.+,-/.).1!89:;4&,!*;_I&*.(A-7+&),L1U$),&,!*;!$6$.#4&3+:;!E!8#!=0,%.$-$E-,)1%.(*123).!$6$.#!$,%.-,.3!*$,!#!3#4&3.!+%(H8,:;+&,!5-!,-&-,%.;(H81%.H8 93-5.3$.,4&3 5!-I.(*123:Q&3!3#:U(AA8A8AAA88(;./.3$.:U(A(88AAA8(1%.H8 93-5.3$.,4&3 4(5/!63

32、:Q&3!3#:U(A8A8A888888A8A8A8A88%.93&)3!5 4&3 H8!$:C V U 5-4&3#.!(,63.;C V J 5-,U V J 5-,XL V J 5-4&3 U+0(+*.$4&3!59*-4-+!,-&;XLV JN 5-4&3.?,.$-&!#$&!-C V 1=9)2&-2=!2%-0.%+%?ABC2)+%D.+/ABC;,.+D.+/7*%;).+2(0*)%/+.E)A9F)-)5)+993&?-5!,.*0 N1 R JNT0.!$,3!$4&35!,$.3.$+3.#6$-)+:;%.9&$-,-/.+*&.$)3.&,%.9*!,.&

33、4=:KO-$WP3!WB.6W+&,!-)LN 55&*KB(.3.$.*.+,.#!#+6*,63.#-2H:5.#-651%.$.*.+,.#+*&.$.3.463,%.3+&4-35.#70 7(a!+,-/-,0!$!0&,%.4-*,.3 9!(9.3$+&,!-)?()!*1 8*&.$4&35-)7*6.+&*&-.$!#3.$-$,!,&LN 55&*KB(.3.+&$-#.3.#4&3 463,%.3.?!5-!,-&1 D0,%-$5.!$.-#.,-4-.#&.9&$-,-/.+*&.%-+%+&6*#)3&.*./.&TN 55&*KB(9*!,.!#%!#!3.*!

34、,-/.%-)%7(a!+,-/-,0&,%.4-*,.31%.+:;43!)5.,&4,%-$+*&.!$.?+-$.#70!#!#*-)!,.#,&9D*6.$+3-9,=EF4&3$.G6.+-)(Q-)1J)1=-+.,%.+:;!$*-)!,.#,&,%.#&$,3.!5&4ABC!+,-/!,-&#&5!-9:(ABC 9%!).(5-#/.+,&3-*-73!30+&$,36+,-&,.+&6*#&*0.?!5-,$7-#-)!(7-*-,0&,%.3,%!+,-/!,-&!7-*-,01 _&3#.3,&463,%.3+&(4-35,%.!+,-/!,-&!7-*-,0&4,%

35、.9&$-,-/.+*&.,.*-)!,.#,%.9&$-,-/.+:;43!)5.,&2.9AH,!0.!$,.?93.$($-&/.+,&3-,%&6,ABC#&5!-1 4,.3,3!$4&35!,-&,,%.0.!$,3.9&3,.3+.*$+&6*#!*$&)3&35!*0&LN,N,CU!#TN 55&*KB(9*!,.$1 Q-)63.J$%&$,%.*8(29-!+,-/!,-&3.$6*,$4&3 5!:ZDJ 93&,.-$-0.!$,+.*1b%.2.9AH9*!$5-#+&,!-)4(5/!63+:;39W-,%.93.$.+.&4 N 55&*KB(9*!,.1O&.

36、/.3,,%.0.!$,+.*+!330-),%.56,!,.#:Z.*.(5.,(5:Z)#-#&,$%&(3.$-$,!+.1 _&3#.3,&5.!$63.,%.!()!*!+,&$-#!$.!+,-/-,0,.,&,%.5/!63:!$,%.9&$-,-/.+&,3&*!#!2.9A%.!()!*!+,&$-#!$.!+,-/-,0!$!0$%&.#,%!,7(a.?93.$-&-,%.0.!$,+.*+&,!-)4(5/!63!#,%.:Z.*.(5.,!$-56*!,.#1 JUJ 4&*#$&4,%.+&,3&*1 c,%.+&,3!30,,%.3.!$&$-)-4-+!,+%!

37、).&4 7(a.?93.$($-&-,%.0.!$,+.*$+&,!-)4(5/!63!#5:Z.*.5.,+&59!3.#,&,%.+&,3&*(!7*.J)1%-$3.$6*,$6).$,$,%!,4(5/!63.+&#-)93+,+&6*#$9.+-4-+!*0 7-#,&:Z.*.5.,!#!+,-/!,.,!3).,).?93.$-&12;1;#b%.:Z.*.5.,!$56,!,.#43&5 88A(5:Z),5!:ZDJ 93&,.-+&6*#&,7-#,&-,!#!()!*!+,&$-#!$.!+,-/-,0!$&,!+,-/!,.#1d_;Q.)&*():_$&*!,

38、-&!#=,36+,63!*3!$+3-9,-&Q!+,&3 43&5 M!-I.(=&(4(9)万方数据!#$%$!#$%&#$()!#*+(+$(,+&-#.+/.#+.+00-1,$%&()*234 5678$-9:.&93./9;+?!3(#+0+)#/0&#+).(#&$($(*A;07B?!3(#+.$*#/0&#+)4+.+C=&00,$?%$+$(#-$D/&.#-2 E(+&/0&#+,0(+-$(#+0+)#.+/&.#-&.+,+&-#.+/.#+.+00-&.,$(*4$0 6FG+#?+0+;+(#(6FG)#.&(-).;+1,&()*,(&-&/-$#$%+(#.

39、0)(!),$%&()*(H)&(I+/J!K%+#.(&-&(+*&#$%+(#.0)(L);0(+-$(#+.$*#.+/&.#-&.+,+&-#.+/.#+.+00-&.,$(*;=#&(#6FG+#?+0+;+(#(;6FG)#.&(-).;+1,#+-&;+#.+/0&-;$-:&()*,(!),$%&()*(H)&(I+/J!K%+#.(L),.+-/+#$%+0,2&()*%-%+M+D/.+-$($(,+&-#.+/.#+.+00-#.&(-).;+4$#$%&()*F+/.#+.-#.&$(-!?*&0&#-$(I+/J!KO 6FGHP!)7;6FGA2WWP R A2PV

40、P2AUU(I+/J!KO$%&()*)76FG2WAT R A2SVQ2PUP(I+/J!KO$%&()*)7;6FGA2UT R A2PVSA2SQUI+/J!K76FGA2WPQ R A2PVUP2AAAI+/J!K7;6FGA2VQ R A2ATSA2QQA&()*,&($%&()*(-#.=#+$(I+/J!K%+#.4+.+#.&(-).;+$(#,+&-#IXWSTP78$-O:.&O.+/.#+.(#&$($(*4$0#,/+6FG+0+;+(#.;=?#&#+6FG+0+;+(#(;6FG),.+-/+#$%+0,2 I+&-#+00-4+.+*.4(&-+?0+#$%+;+

41、$=;)5678$-9:.&93./9).SA((Y V)2!,;$00+.=($#-;R,-#&(&.+.2+$+,-./0-/.)1)233 45-6*!#$%&0789Z+-+C=+(+#+$(-+.#+6!).&*;+(#)P2 W12#-4+#&#$%&()*6!(#&$(+&-$(*0+/+(.+&$(*).&;+)SQP&;$(&$-&(+(+&/=?#&#$%+/.#+$(4$#&/.+$#+A2 6(_$*2 S)2 3+-+C=+(+4&-=1;$#+#+J+(H&(&(#+&+-$(=;1+.$-!_WWQTQ2Z+&0-+&.+6!&(/.#+$(&#&1&-+).-+

42、C=+(+;0?*+-#+;&6FGHP/.#+$(-&()=(#&#$-/.#+$(&(-+.%+6!?1$($(*;&$()UQ&;$(&$-/.+-+(#+$(!KS7GFGHK#.&(-.$/#$()&#.2 3+!KS;&$(&0$*(;+(#4$#6FGH/.#+$(-).;,.%/012#-=*+-#+#&#$-6!+(+&6FGH?0$+/.#+$(,-4+(&;+)2H=#+=+&;$(&$-+C=+(+);&6FGHP=#)!KS;&$(-4+(-$*($)$&(#-+?C=+(+$+(#$#,4$4&-&0-1-+.%+&;(*#+.6FGH/.#+$(-2 84+%+.

43、,-$;$0&.#+.6FGH#.&(?-.$/#$()&#.-,;&6FGHP/.#+$(#&$(+&1&-$.+?*$($($#-?#+.;$(&0.+*$(#&#;$*#)=(#$(&-&(=?0+&.0&0$&#$(-$*(&0&(&(&$L?#+.;$(&0.+*$(#&#;$*#&#&-&(&#$%&#$(;&$().#.&(-.$/#$(2!00#+.+-=0#-4+#&#$%&()*+($(*&6!?1$(Q&(5=$V,4&-+;/0,+2 J+?(;$6!4&-$*+-#+4$#3#4,(+1F&(5/%1+).+#.&(-)+.$(*#+;+;1.&(+2 3+,?1.$

44、&#$(4&-&.$+=#=(+.$*?-#.$(*+(,($?#$(-1,=-$(*#+)=00?0+(*#)$%&()*&-/.1+2:(?+.#$-($#$(0,(+.#4,1.$1&(-=0 1+1#&$(+(_$*2W),4$($&#+#&#+$#+.$#+D$-#+&-&04?/,?(=;1+.*+(+./,-$&00,0$(+&a&+(#0,#+&#+.$(.+/+ 3$-/+(;+(4&-&0-$+(#$?)$+$(,.%/012#)6)7 8&()*+(+-(J$0;=.94%:,PQ)2+$;4 0413*.?*=14 03 19+4=1(.+.#&-+-#+)=(#

45、$(&0-$*($)$&(+)#4&1-0=#+0,(-+.%+.+-$=+-&#+PW#b&0&(#+P#J0=,$(!KS;&$();&6FGHP,4+;=#&#+#+-+#4&;$(&$-$(#!0&(!-/.+-/+#$%+0,&(-$;=0#&(+?=-0,2!-4+(4 b&0&(J0=&%+-$;$0&.$+(#$#,4$#!0&(!-/1#$(,./$0$#,&(&$#,2)#$-$();=#&#$(0+&-#*.+&#&(*+-$(6!?1$($(*&#$%$#,)#+/.#+$($#;$*#-=*+-#&#+-+#4.+-$=+-&%+*.+&#-$*($)$&(+$(6!

46、?1$($(*&#$%$#,&(&(,-=1-#$#=#$()#+;4$00 0+&#*.+�-)#+1$($(*&#$%$#,2 3.+;=#&(#6!-4+.+.+-/+#$%+0,0$*&#+#I+/J!K+D/.+-$(%+#.,&0(*4$#+4$0?#,/+$%&()*,#.&(-).;+#,+&-#.+/.#+.-#.&$(&.?.,$(*4$0?#,/+6FG+0+;+(#,.+-/+#$%+0,2 _=.#.&(-).?;&(#-$(4$0?#,/+6FG+0+;+(#.+/.#+.+00*.+4(5678$-9:.&93./9;+?!3,1=#+#.&(-).;&(#-

47、4$#$%&()*.+4 1+#+.#&(#+#+.#.+2 E(0,;=#&(#XP$(W植物学报,+4%)14%;+%SAA万方数据!#$%$!#$#%&()*%+%&,%-.!#$%&,/0123)%454678179:-;=&*%&:%#=&%:2 3)%?%=-&=&()%0$(%;=&%=-&()(;=)(.&,(=-&*&,#%#-,#=(=-&*=&#=&%:2 3)%=*&;=&%=-&()(;=)(,(*=B(=-&#,(=C(=-&:-;=&2 3)=*%+%&,%)*?%&*?;=(%:(-()%D%&6&E:(?*%F=()()%1!GGHIHJ2!#$&$3)%17

48、9:-;=&#=&;%&(-.;/546 B-(%=&*,*,)*/546K1(16LLIIHI),/546K6(16LLIIHH),/546KA(16LLIIHJ),/54691(16LLIIJL),/54696(16LLIIJK),3M0N(OJGPJH),51792 K(1NLHPHQH)=&()*+,-./+/;1A54KKK1(1!9KKRQL2K),1A54KKK6(1!9KKRQK2K)=&0+1-2+3 2*14!;A6!I(1!GJJLQ92K),A6!KP(1!GJJLQQ2K)=&)/+13.4/5 3)%?#,E?-&:%B%*%&(*B%.%,(#S,-&*%C%:;

49、=&-%*=:%*2 3)%B(=C%:%*=:%*%*)C%:;=&-,=:KGT&:KJ4%=*E%:2UM0!%&62 7:M*-#(=-&,(#1&=B(=-&!.-;W=%(86!9/)QQR万方数据!#$%$!#$%&()*$+(,-(-*./+/0,&(1+2/&3#&(2&/%1*4,#/0!#$%&256789%&,&(1+2 567 0$:,&($.;&0 1-+?($%&$%&*-(&/+(*&0$)-(A!%#+B($%&$%&*-(&/+(+,%$),:&A+,&/$&A:+$%()C!(D),*+!(E)-(A,-.(=)8 9%&0#*4*&(,$%!#$%&25

50、67:-/#/&A-/-;)&89%&(#1)&/-$+,%$-&1*&2#*-*&(,$%1-F&/+(F+*)-/&/8:+*A4$.;&5CD&;$&2&*2#*A,:(?G 11*HI?479;*-$&8 7*0$%&?1#$-($/2#*A($,:(JG11*HI?479;*-$&,%:&K&,!#$%&$-(/01-($/,&:&*#(A&$%+/2(A+$+(84,-*-2$/+A-/&-2$+K+$.-/-./%:&A$%-$:%&(L-*:-/2%-(,&A+($7*-$%&J$%,$%&*-$+K&(M8NO 8 P%&(Q*#:-/2%-(,&A+(7/;-$%&N$%,

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