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1、精选学习资料 - - - - - - - - - PCR设计引物时酶切位点的爱护碱基表名师归纳总结 酶寡核苷酸序列切割率 % 第 1 页,共 5 页Acc I GGTCGAC C 2 hr 20 hr 00CGGTCGAC CG 00CCGGTCGAC CGG 00Afl III CACATGT G 00CCACATGT GG 9090CCCACATGT GGG 9090Asc I GGCGCGCC9090AGGCGCGCC T 9090TT GGCGCGCC AA 9090Ava I CCCCGGG G 5090CCCCCGGG GG 9090TCCCCCGGG GGA 9090BamH I
2、 CGGATCC G 1025CGGGATCC CG 90909090CGCGGATCC GCG Bgl II CAGATCT G 00GAAGATCT TC 7590GGA AGATCT TCC 2590BssH II GGCGCGC C 00AG GCGCGC CT 00TTG GCGCGC CAA 5090BstE II GGGTA/TACC C 010AACTGCAGAACCAATGCATTGG00BstX I AAAACTGCAGCCAATGCATTGG AA 2550CTGCAGAA CCAATGCATTGG ATGCAT 2590CATCGAT G 00Cla I GATCGAT
3、 C 00CCATCGAT GG 9090EcoR I CCCATCGAT GGG 5050GGAATTC C 9090CGGAATTC CG 9090CCGGAATTC CGG 9090- - - - - - -精选学习资料 - - - - - - - - - GGGGCCCC 9090Hae III AGC GGCCGCT 9090TTGC GGCCGCAA 9090Hind III CAAGCTT G 00CCAAGCTT GG 00CCCAAGCTT GGG 1075Kpn I GGGTACC C 00GGGGTACC CC 9090CGGGGTACC CCG 9090Mlu I 00
4、GACGCGT C CGACGCGT CG 2550Nco I CCCATGG G 00CATG CCATGG CATG 5075Nde I CCATATG G 00CCCATATG GG 00CGCCATATG GCG 00GGGTTT CATATG AAACCC 00Nhe I GGAATTC CATATG GAATTCC 7590GGGAATTC CATATG GAATTCCC 7590GGCTAGC C 00CGGCTAGC CG 1025CTA GCTAGC TAG 1050 TT GCGGCCGC AA 00Not I ATTT GCGGCCGC TTTA 1010AAATAT G
5、CGGCCGC TATAAA 1010ATAAGAATGCGGCCGC TAAACTAT 2590AAGGAAAAAAGCGGCCGC AAAAGGAA2590AA 名师归纳总结 Nsi I TGCATGCAT GCA 1090第 2 页,共 5 页9090CCA ATGCAT TGGTTCTGCAGTT Pac I TTAATTAA00GTTAATTAAC 025CCTTAATTAAGG 090GTTTAAAC00Pme I GGTTTAAAC C 025GGGTTTAAAC CC 050AGCTTT GTTTAAAC GGCGCGCCGG 7590- - - - - - -精选学习资料 -
6、 - - - - - - - - GCTGCAG C 00Pst I TGCA CTGCAG TGCA 1010AA CTGCAG AACCAATGCATTGG 9090AAAA CTGCAG CCAATGCATTGGAA 9090CTGCAG AACCAATGCATTGGATGCAT 00Pvu I CCGATCG G 00AT CGATCG AT 1025TCG CGATCG CGA 010Sac I 1010CGAGCTC G GCCGCGG C 00Sac II 5090TCCCCGCGG GGA Sal I GTCGAC GTCAAAAGGCCATAGCGGCCG00C 1050GC
7、GTCGAC GTCTTGGCCATAGCGGCCGC1075GG ACGC GTCGAC GTCGGCCATAGCGGCCGCGGAA 名师归纳总结 Sca I GAGTACT C 1025第 3 页,共 5 页7575AAA AGTACT TTT Sma I CCCGGG010CCCCGGG G 010CCCCCGGG GG 1050Spe I TCCCCCGGG GGA 9090GACTAGT C 1090GGACTAGT CC 1090CGGACTAGT CCG 050Sph I CTAG ACTAGT CTAG 050GGCATGC C 00CAT GCATGC ATG 025ACA
8、T GCATGC ATGT 1050Stu I A AGGCCT T 9090GA AGGCCT TC 9090AAA AGGCCT TTT 9090Xba I CTCTAGA G 00GCTCTAGA GC 9090TGCTCTAGA GCA 7590- - - - - - -精选学习资料 - - - - - - - - - CTAG TCTAGA CTAG 7590CCTCGAG G 00Xho I CCCTCGAG GG )1025CCGCTCGAG CGG 1075CCCCGGG G 00Xma I CCCCCGGG GG CCCCCCGGG GGG 25755090TCCC CCCG
9、GG GGGA 9090ApaI (类型: Type II restriction enzyme 识别序列:5GGGCCC 3BamHI (类型: Type II restriction enzyme )识别序列: 5 GGATCC 3BglII (类型: Type II restriction enzyme )识别序列:5 AGATCT 3EcoRI (类型: Type II restriction enzyme )识别序列:5 GAATTC 3HindIII (类型: Type II restriction enzyme )识别序列:5 AAGCTT 3KpnI (类型: Type II
10、restriction enzyme )识别序列:5 GGTACC 3NcoI (类型: Type II restriction enzyme )识别序列:5 CCATGG 3NdeI (类型: Type II restriction enzyme )识别序列:5 CATATG 3NheI (类型: Type II restriction enzyme )识别序列:5 GCTAGC 3NotI (类型: Type II restriction enzyme )识别序列:5 GCGGCCGC 3名师归纳总结 - - - - - - -第 4 页,共 5 页精选学习资料 - - - - - - -
11、 - - SacI (类型: Type II restriction enzyme )识别序列:5 GAGCTC 3SalI (类型: Type II restriction enzyme )识别序列:5 GTCGAC 3 SphI (类型: Type II restriction enzyme )识别序列:5 GCATGC 3XbaI (类型: Type II restriction enzyme )识别序列:5 TCTAGA 3XhoI (类型: Type II restriction enzyme )识别序列:5 CTCGAG 3 名师归纳总结 - - - - - - -第 5 页,共 5 页