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1、PCR 设计引物时酶切位点的保护碱基表切割率 % 酶寡核苷酸序列2 hr 20 hr Acc I GGTCGAC C CGGTCGAC CG CCGGTCGAC CGG 000000Afl III CACATGT G CCACATGT GG CCCACATGT GGG 0909009090Asc I GGCGCGCCAGGCGCGCC T TTGGCGCGCC AA 909090909090Ava I CCCCGGGG CCCCCGGG GG TCCCCCGGG GGA 509090909090BamH I CGGATCC G CGGGATCC CG CGCGGATCC GCG 1090902
2、59090Bgl II CAGATCT G GAAGATCT TC GGAAGATCT TCC 0752509090BssH II GGCGCGCC AGGCGCGCCT TTG GCGCGC CAA 00500090BstE II GGGT(A/T)ACC C 010BstX I AACTGCAGAACCAATGCATTGGAAAACTGCAGCCAATGCATTGG AA CTGCAGAA CCAATGCATTGG ATGCAT 0252505090Cla I CATCGAT G GATCGAT C CCATCGAT GG CCCATCGAT GGG 009050009050EcoR I
3、GGAATTC C CGGAATTC CG CCGGAATTC CGG 909090909090精选学习资料 - - - - - - - - - 名师归纳总结 - - - - - - -第 1 页,共 5 页Hae III GGGGCCCC AGCGGCCGCT TTGC GGCCGCAA 909090909090Hind III CAAGCTT G CCAAGCTT GG CCCAAGCTT GGG 00100075Kpn I GGGTACC C GGGGTACC CC CGGGGTACC CCG 0909009090Mlu I GACGCGT C CGACGCGT CG 025050Nco
4、 I CCCATGG G CATG CCATGG CATG 050075Nde I CCATATG G CCCATATG GG CGCCATATG GCG GGGTTT CATATG AAACCC GGAATTC CATATG GAATTCC GGGAATTC CATATG GAATTCCC 0000757500009090Nhe I GGCTAGC C CGGCTAGC CG CTA GCTAGC TAG 0101002550 Not I TTGCGGCCGC AA ATTT GCGGCCGC TTTA AAATAT GCGGCCGC TATAAA ATAAGAATGCGGCCGC TAAA
5、CTAT AAGGAAAAAAGCGGCCGC AAAAGGAAAA 010102525010109090Nsi I TGCATGCAT GCA CCA ATGCAT TGGTTCTGCAGTT 10909090Pac I TTAATTAAGTTAATTAAC CCTTAATTAAGG 00002590Pme I GTTTAAACGGTTTAAAC C GGGTTTAAAC CC AGCTTT GTTTAAAC GGCGCGCCGG 000750255090精选学习资料 - - - - - - - - - 名师归纳总结 - - - - - - -第 2 页,共 5 页Pst I GCTGCAG
6、 C TGCA CTGCAG TGCA AACTGCAG AACCAATGCATTGG AAAA CTGCAG CCAATGCATTGGAA CTGCAG AACCAATGCATTGGATGCAT 0109090001090900Pvu I CCGATCG G ATCGATCG AT TCGCGATCG CGA 010002510Sac I CGAGCTC G 1010Sac II GCCGCGGC TCCCCGCGG GGA 050090Sal I GTCGAC GTCAAAAGGCCATAGCGGCCGC GCGTCGAC GTCTTGGCCATAGCGGCCGCGG ACGC GTCGA
7、C GTCGGCCATAGCGGCCGCGGAA 0101005075Sca I GAGTACT C AAA AGTACT TTT 10752575Sma I CCCGGGCCCCGGGG CCCCCGGG GG TCCCCCGGG GGA 00109010105090Spe I GACTAGT C GGACTAGT CC CGGACTAGT CCG CTAG ACTAGT CTAG 10100090905050Sph I GGCATGC C CAT GCATGC ATG ACAT GCATGC ATGT 001002550Stu I A AGGCCT T GAAGGCCT TC AAA AG
8、GCCT TTT 909090909090Xba I CTCTAGA G GCTCTAGA GC TGCTCTAGA GCA 0907509090精选学习资料 - - - - - - - - - 名师归纳总结 - - - - - - -第 3 页,共 5 页CTAG TCTAGA CTAG 7590Xho I CCTCGAG G CCCTCGAG GG CCGCTCGAG CGG 0101002575Xma I CCCCGGGG CCCCCGGG GG CCCCCCGGG GGG TCCCCCCGGG GGGA 02550900759090ApaI (类型: Type II restricti
9、on enzyme )识别序列:5GGGCCC 3BamHI (类型: Type II restriction enzyme )识别序列: 5 GGATCC 3BglII (类型: Type II restriction enzyme )识别序列:5 AGATCT 3EcoRI (类型: Type II restriction enzyme )识别序列:5 GAATTC 3HindIII (类型: Type II restriction enzyme )识别序列:5 AAGCTT 3KpnI (类型: Type II restriction enzyme )识别序列:5 GGTACC 3Nco
10、I (类型: Type II restriction enzyme )识别序列:5 CCATGG 3NdeI (类型: Type II restriction enzyme )识别序列:5 CATATG 3NheI (类型: Type II restriction enzyme )识别序列:5 GCTAGC 3NotI (类型: Type II restriction enzyme )识别序列:5 GCGGCCGC 3精选学习资料 - - - - - - - - - 名师归纳总结 - - - - - - -第 4 页,共 5 页SacI (类型: Type II restriction enzyme )识别序列:5 GAGCTC 3SalI (类型: Type II restriction enzyme )识别序列:5 GTCGAC 3 SphI (类型: Type II restriction enzyme )识别序列:5 GCATGC 3XbaI (类型: Type II restriction enzyme )识别序列:5 TCTAGA 3XhoI (类型: Type II restriction enzyme )识别序列:5 CTCGAG 3 精选学习资料 - - - - - - - - - 名师归纳总结 - - - - - - -第 5 页,共 5 页