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1、-WPM培养基的配制-第 2 页WPM培养基母液配方名称成分含量(mg/L)单位母液加入药品(g)0.5L1.0L1.5L2.0L大量K2SO49909.919.829.739.6MgSO47H2O3703.77.411.114.8KH2PO41701.73.45.16.8NH4NO34004.081216微量MnSO44H2O22.52.254.56.759ZnSO47H2O8.60.861.722.583.44H3BO36.20.621.241.862.48CuSO45H2O0.250.0250.050.0750.1Na2MoO42H2O0.250.0250.050.0750.1钙盐Ca(
2、NO3)24H2O55627.855.683.4111.2铁盐FeSO47H2O27.82.785.568.3411.12Na2EDTA37.33.737.4611.1914.92有机物肌醇1002468甘氨酸2.00.20.40.60.8VB11.00.10.20.30.4VB60.50.050.10.150.2VB50.50.050.10.150.2注: 此处含量为最终WPM培养基中的含量。 先将Na2EDTA用去离子水溶解,加热至沸腾23次,再用一个瓶子溶好铁盐,待Na2EDTA完全冷却以后,再将铁盐倒入其中并混匀。由于铁盐比较容易结晶,装铁盐的瓶子一定要用去离子水清洗干净,并放在烘干箱中烘干。WPM培养基配方名称每单位培养基须加入母液量(mL)0.25L0.5L1.0L2.0L3.0L4.0L大量12.52550100150200钙盐2.5510203040微量1.252.55101520铁盐1.252.55101520肌醇1.252.55101520甘氨酸0.250.51234VB10.250.51234VB60.250.51234VB50.250.51234注:琼脂和蔗糖按需要添加,木本植物可按5g琼脂+20g蔗糖/L比例配制。