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1、Effect of HANS on heart rate of heroin addictsDay of treatment1234567891060708090100110120130Control2 Hz2/100 Hz100 Hzn=13-16*Heart Rate(beat/min)第1页/共36页Body Weight(kg)01234567891011Days of Treatment45505560Control2Hz2/100Hz100Hz(n=26-32)Effect of HANS on Body Weight during Opiate Withdrawal*第2页/共3
2、6页The dose of The dose of BPNBPN needed by Heroin Addicts needed by Heroin Addicts during detoxification during detoxificationBPN Dose(BPN Dose(mg/dmg/d)1 12 23 34 45 56 67 78 89 91010 11111212 1313 1414Days of Treatment Days of Treatment (n=14)(n=14)0.00.00.50.51.01.01.51.52.02.02.52.5*Buprenorphin
3、eBuprenorphine HANS+BPN HANS+BPN *P0.001,compared with BPN group *P0.001,compared with BPN group 第3页/共36页CRAVING:the most difficult issue一朝吸毒，终身想毒。第4页/共36页 1 Month 1 Month0 05050100100Relapse Rate(%)Relapse Rate(%)*P0.05,compared with BPN group *P0.05,compared with BPN group 92%92%78%78%57%57%71%71%
4、100%100%100%100%HANS Decreases Heroin Relapse Rate of the Heroin addicts 2 Month 2 Month 3 Month 3 MonthBPN(n=12)BPN(n=12)HANS+BPN(n=14)HANS+BPN(n=14)*第5页/共36页The auto-track conditioned place preference(CPP)instrument第6页/共36页Morphine-induced conditioned place preference(CPP)Dose-dependent effect*p0.
5、05,*p0.01,*p 100 Hz100 Hz 2 Hz第13页/共36页Electroacupuncture treatment for drug addictionVTAOpiateGABANAcDADATCocaineEuphoriaEnkDynElectroacupuncture2 Hz100 HzPresynaptic inhibitionSubstitution第14页/共36页A100 bp2000 bp1000 bp750 bp500 bp250 bpDL 2000 NSCPP2 Hz100 HzrestrainGAPDH(532 bp)PPD(250 bp)Effect
6、of 100Hz EA on the level of PPD mRNA in NAc of CPP rats B0.00.51.01.5Grey scale ratios(PPD/GAPDH)Treatment*#n=4NSCPP2 Hz100 Hzrestrain第15页/共36页Effect of 2Hz EA on the level of PPE mRNA in NAc of CPP rats Grey scale ratios(PPE/GAPDH)0.000.250.500.751.00NSCPP*#2 Hz100 Hzrestrainn=42000 bp1000 bp750 bp
7、500 bp250 bp100 bpDL2000CPP2 Hz100 HzrestrainGAPDH（532 bp）PPE（402 bp）NS第16页/共36页美国权威性“物质滥用”教科书Joyce Lowinson 等主编第4版，2004“针刺戒毒”一章由韩济生编写第17页/共36页肥肥 胖胖 症症能量代谢失衡能量代谢失衡对美食的渴求对美食的渴求药物成瘾药物成瘾身体依赖：戒断症状身体依赖：戒断症状精神依赖：心瘾精神依赖：心瘾痛与镇痛痛与镇痛感知功能感知功能情绪反应情绪反应第18页/共36页肥胖的发展趋势肥胖的发展趋势第19页/共36页肥胖发病机理GenesEnvironmental fact
8、ors 第20页/共36页HANSHANS治疗肥胖症：人体研究结果治疗肥胖症：人体研究结果048121620242832-4-3-2-10 A HANS B C HANS Dn=16Time(week)ABCD-4-3-2-10*HANS(3 time/week)Change of body weight(kg)梁门，一对。天枢，一对。水道，一对。梁丘，公孙。频率：2/100 Hz每周3次每次30 分钟第21页/共36页 大鼠实验结果（高能食物引起肥胖）大鼠实验结果（高能食物引起肥胖）Diet-induced Obesity(DIO)After 14 weeks0246810121416010
9、0200300400500600700High energy diet(n=80)Normal diet(n=20)*Time(week)Body weight(g)ControlDRDIO0100200300400500600700800*#High energy dietBody weight(g)*P0.001 vs.control and DR;#P0.05 vs.Control第22页/共36页促进摄食和抑制摄食的神经肽促进摄食 feedingNPY,Orexin,AGRP,MCH抑制摄食 feedingLeptin,CART,a-MSH,CRHNPYOrexinAGRPMCHCAR
10、TLeptin-MSH Insulin瘦胖ThinObese第23页/共36页100Hz036912151821242730Time(day)9092949698100102104106108110Body weight (%)DIO大鼠实验结果（体重大鼠实验结果（体重%）Restrain2Hz第24页/共36页大鼠实验结果（体重差值）大鼠实验结果（体重差值）*P0.001 vs.DIO;#p0.01,#P0.001 vs.RestrictionDIORestrain100 Hz2 Hz第25页/共36页大鼠实验结果（大鼠实验结果（ACTHACTH）Normal DR DIO Restrain
11、 100Hz EA 2Hz EA051015202530*High energy diet*Plasma ACTH (ng/ml)*P0.05 vs.Normal,DR and DIO.第26页/共36页 大鼠实验结果（能量摄取）大鼠实验结果（能量摄取）*P0.001 vs.other groups;#P0.001 vs.2Hz and 100 Hz EA group 第27页/共36页 CART -MSH第28页/共36页ControlDiet induced ObesityDiet ResistantRestrain100Hz2HzCART immunoreactivity in hypo
12、thalamus第29页/共36页ControlDiet induced ObesityDiet ResistantRestrain100 Hz 2Hz-MSH immunoreactivity in hypothalamus第30页/共36页大鼠实验结果（免疫组化）大鼠实验结果（免疫组化）n=5NormalDRDIORestrain100Hz EA0255075100125 *High energy diet CART positive cells in ARCHigh energy dietNormalDRDIORestrain100Hz EA2Hz EA010203040506070*a
13、-MSH positive cells in ARC *2Hz EACART-MSH第31页/共36页 国际神经科学神经科学领域：英国TiNS杂志（2003）国际针灸针灸领域：德国SEIRIN针灸奖（2002）国际戒毒戒毒理论：美国“物质成瘾”经典教科书 临床戒毒戒毒实践：韩氏戒毒一年以上不复吸20例36年针灸研究，12年戒毒研究所获成绩第32页/共36页Trends in Neuroscience,2003;26:17-22 第33页/共36页International SIERIN Acupuncture Award 2002Dusseldorf,Germany 第34页/共36页美国权威性“物质滥用”教科书Joyce Lowinson等主编第4版，2004“针刺戒毒”一章由韩济生编写第35页/共36页感谢您的观看！第36页/共36页