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1、PureSpect Manual(English version)PureSpect User Support CenterSAIKA Technological Institute FoundationT640-8390 75-2 Kuroda, Wakayama-city JAPANTEL +81-73-474-0860FAX +81-73-474-0862URLE-mail kwonsaika.or.jpReference gainThe Reference gain“ command is a parameter which sets a reference gain in the m
2、anual mode. The range of a reference gain is from 0 to 255.Num. of 1st PV read-outThe “Num. of 1st PV read-out is a number of the CCD diode array sensor to be used in the AutoGain area. The range of the Num. of 1st PV read-out is from 1 to 127.2.7. MA & Auto GainAuto gain positionThe “Auto gain posi
3、tion9 indicates a position of an index line in the Auto gain area. You can move a position of an index line where you want to locate. If you want to display the index line, check a box next to the green color button of the left side. You can also lock the index line checking a box which is located a
4、t the right side.MP& Auto gainGain constant displayAuto gain position， Gain constant display D line 469The “Gain constant display? indicates a position of an index line of aMP switchAuto gain SUM number0Gain constant. The Auto gain makes a target spectrum amplify with a vertical axis toward this lin
5、e. If you want to display this index line, check a box next to the yellow color button of the left side.Auto gain gain200 jJfg jJTTargetOthersGain0 65519Size057341Speed030591Number01Exp. time02558.35ESize065469CloseRing buffer 0Flag 4D lineThe “D line“ indicates a value of a spectrum which is measur
6、ed in the dark state. The value of the D line is indicated at the right side of the 3rd line in the MP & Auto gain window. If you want to display a D line spectrum, check a box next to the gray color button of the left side.MP switchThe “MP switch“ command is a channel to communicate data which cons
7、ist of a target, a reference or an offset etc. You can select a channel of data if you move this scroll bar.Auto gain SUM numberThe “Auto gain SUM number55 indicates how many data you use for the auto gain. The range of this parameter is from 1 to 253.Auto gain gainThe “Auto gain gain“ command is a
8、parameter which sets a gain of auto gain. The range of an auto gain gain is from 0 to 255. If you want to display a spectrum of an auto gain, check a box next to the scroll bar in the left side.Status windowThe “Ring buffer“ is a memory buffer to load the data of a spectrum. It consist of the 4 bloc
9、ks, is rotated from 0 to 3 automatically. The Flag” is one of the inner circle channels in the spectrometer.It is mapped as a target is 1, a reference is 2, an offset is 3 and a D line is 4. The “Target” and “Others” columns indicate a state of a reference or an offset state. The Gain” is a value of
10、 a gain in the target and reference (or offset) mode. The “Size” is a size of the target as the 0.1mm unit. The “Speed“ is a speed of the turn table as 0.5mm/sec unit. The “Number“ is a counter of the target which is reset if you click the button located left bottom side. The “Exp. time“ is a exposu
11、re time of target. The “E Size” dont use in this research project.28 Motor & LampMotor Stop positionThis parameter cannot be used and neednt in this research project. It has to be installed an elevation motor in the lamp housing which is used the on-line packing house system.Turn TableThe “Turn Tabl
12、e“ command is used when you want to operate the Fruits-analyzer. If you click ON” button, the turn table of the Fruits-analyzer starts to be rounded measuring a spectrum. You can find a speed value of the turn table in the right side of this command. The range of the speed of the turn table is from
13、Om/min to 90m/min (from 0 to 3000 pulse). But you have to calculate a speed value from pulse to mm/second,because, its value is displayed as pulse unit. For example, if it is displayed 100, the speed of turn table is 50mm/second.Lampl (or Lamp2)This command is used when the lampl (or lamp2)is turned
14、 on or off. You can control the light level of lampl (or lamp2)using the scroll bar in the Lampl (or Lamp2)command. The range of the light level is from 0 to 10. If you set the light level 10, the lampl (or lamp2)is set full level of 100 watt.Lamp3, 4The command cannot be used and neednt in this res
15、earch project.3. Calculation3 J. Edit dataTable I.The data sheet of NIR spectra.If you open a spectrum file using the EXCEL software, you can load a data sheet of spectra. (Table 1) The spectrum data is only from the 15th line to the 274th line in the full 600 lines. But you had better use only from
16、 15th line to the 269th line to the edit data in the EXCEL software. The meaning of the value of the 15th line is the value of the spectrum in the 643.26nm. The values from the 1st line to the 14th line are meaning of target mode, a value of gain, a size, a speed of the turn table, a number, a targe
17、t exposure time, a dummy size, a dummy value 1 - 7, respectively. In Table 2, you can find the data edited from the Table 1. The data in the Table 2 are the spectra of water which is changed by temperature conditions.The data of spectra in the Table 3 which is the absorbance spectra of water are cal
18、culated from Table 2 using a follow simple equation.11111200003818981898189818941625516255162551625551111620020020020071628516285162851628580000930253054303930271019671988196219881158558758858212620612623608136346356256351470972371471915182018111815181516183718361834184417185518601851183918187818771
19、8601864191882189318881895201912190819051909No. w ater-100 w ater-90C w atjer-80C w ater-70C4497 7 2 8 14 7 9 149 9 9 0 0203021794 0 7 7 55 7 8 149 9 9 0 01112 290724995499 9 0 0 0112 2 22 2 10 8 126 8 13 6 139 9 0 0 0 11112 2 2 2 2216521552162, / Reference - D line、: log()Target - D lineTable 2. The
20、 data sheet of raw spectra in the water which is changed by temperature conditions643.26644.55645.88647.21648.54649.87651.2652.53653.9water-100C182018371855187818821912194419471977water-90C181118361860187718931908193719541970water-80C181518341851186018881905192719491966water-70C181518441839186418951
21、909192819541962water-60C181718301851185418841901191919431970water-50c179318351850186718841891191419481957water-40C179618181844184518791897191919291952water-30C178918341827184218731899190619321946Reference158615951618163116401659167216961714D line470470470470470470470470470Table 3. The data sheet of
22、absorbance spectra in the water which is changed by temperature conditions.643.26644.59645.92647.25648.58649.91651.24652.57653.9water-100C-0.083-0.085-0.082-0.084-0.082-0.084-0.089-0.081-0.08water-90C-0.08-0.084-0.083-0.083-0.085-0.083-0.087-0.083-0.08water-80C-0.081-0.084-0.08-0.078-0.083-0.082-0.0
23、84-0.081-0.08water-70C-0.081-0.087-0.076-0.079-0.086-0.083-0.084-0.083-0.08water-60C-0.082-0.082-0.08-0.076-0.082-0.08-0.081-0.08-0.08water-50C-0.074-0.084-0.08-0.08-0.082-0.077-0.08-0.081-0.08water-40C-0.075-0.079-0.078-0.073-0.081-0.079-0.081-0.076-0.08water-30C-0.073-0.084-0.073-0.073-0.079-0.08-
24、0.077-0.076-0.0732 View SpectraThe fact is that the explanation of usage of the PureSpect software finished in this stage, because you will get to use the “The Unscrambler55 application software from this step. If you have already gained experience in working with “The Unscrambler9 software, it will
25、 be unnecessary to explain the next step. By the way, if you import the EXCEL file which has the data sheet of the absorbance spectra in the “The Unscrambler” software, you must be able to draw the spectra on the “The Uncrambler“ window and proceed to future steps.1 . OverveiwThis chapter introduces
26、 the general information on the PureSpect operation software. The PureSpect operation software consists of the main menu of six windows as the below figure.1. Continuation save : The window is to save a spectrum.2. Sampling : The window is to operate various sampling functions.3. Add index line : Th
27、e window is to set various index lines in the spectrum window.4. Reading time : The window is to control measuring conditions.5. MP & Auto gain : The window is to overview measuring status.ED回区I6. Motor & lamp : The window is to control lamps on/off etc.lsntst40.exe Parameter-SPC With no offset comp
28、ensationFile(F)Para meter (P)DisplayRT L MPRT L MP-la|x|FolderCommon file name Ext.Continuation saveSampling浑Program Fileslsn45|.| WAVEixf|WAVE000.TKTr o AUTOC 1 TargetC 2 Offset3 Reference6 4 D line Continuationp AutoGain Overwrite Target only No display Save Ref. displaySamplingAdd index lineX=107
29、H .2900056MP& Auto gainAuto gain position*Gain constant display D line 469MP switchAuto gain SUM number9QOI Target(OthersGain0 65519Size057341I Speed030591Number01Exp. time02558.35E Size065469IiCloseAuto gain gain 200 - |Ring buffer 0Flag 4mpling intern10FileThe File menu consists of Save”, “Extensi
30、on Mode” and Exit” commands. The Save” command is used when you save the spectra which is measured. The Extension Mode“ command is used when the PureSpect is needed a troubleshooting. The “Exit” command is used when you close the PureSpect software.ParameterThe Parameter menu consists of Automatic p
31、arameter measurement” and “Motor and lamp control” commands. The uAutomatic parameter measurement55 command is used when you want to check the conditions of a CCD diode array sensor. The “Motor and lamp control” command is used when you control lamps or a turn table.DisplayThe Display menu consists
32、of Drawing line” and Error display commands. The “Drawing line” command is used when you want to draw index lines in the spectrum window. The “Error display” command is used when you check error messages which are occurred by a trouble in the PureSpect software or instrument.2. Operation2A. Select p
33、arameterThe “setprm2 icon is displayed in the Windowsdesktopmenu.Click on this icon to start thePureSpect software.If the login dialog box is opened, select “Parameter file“ in the drop-down list. The name of parameter file is a 6tParameter-SPC?, for your spectrometer. A Saika TIF service technician
34、 has configured your copy of the PureSpect software to match your spectrometer when your spectrometer was installed.Click on the PureSpect start” button and the PureSpect main window opens.se电Eye，-万夕二人NewIf you want to create a new parameter file, click the “New” icon and the COPY window opens. You
35、can write a new parameter file in the entry box. This menu is useful when you create a new parameter file which is similar to a former file.Phase checkYou can check communication between a computer and the PureSpect instrument, when the PureSpect software will be started. If the icon is clicked, the
36、 PureSpect software will start after checking whether a communication between the computer and the PureSpect instrument is working well or not. In this case, you can find “Under a communication test” message on your screen during a few seconds.V Z t e + 双Under a communication testOffset file reading
37、If it is clicked, it reads a “Offset file“ while starting the program. The offset file is the data file of spectrum which was saved when the CCD diode array sensor turned on. If you start the PureSpect software without the offset file, you have to pass a calculating step which corrects the Offset fi
38、le whenooooooCancela spectrum is converted to an absorbance spectrum for future procedures. Please, click this icon toread the offset file.ChangeIf you changed “Phase check” or “Offset file reading” command, click this icon to save a changing condition. If the change button is displayed in gray, the
39、 conditions were saved.Open folderUse this command to read parameter file.2.2. Spectrum WindowWhen loading the PureSpect software, the spectrum window is displayed in the screen with “Sampling” and MP & Auto gain” windows. The spectrum window consists of the “title bar”, “pulldown menu”, “icons”, “s
40、pectrum display window” and status bar”. The title bar shows the execution file name, parameter file name and status whether the offset file is read or not. The pull-down menus can be used to access all PureSpect functions. The icons may be configured to quickly access frequently used functions. If
41、you move the mouse cursor on an icon, a small text is displayed to explain its meaning. The status bar indicates the cursor position, the wavelength and temperature of the CCD diode array sensor. The default settings for the spectral range are from 643.26nm to 985.11nm with 1.29nm increment.2.3. Con
42、tinuation SaveYou can save measured spectra as follows. At first, select your folder in the Folder box and input a common file name in the “Common file name box. The next step, click the Save” icon, after you finished to measure a spectrum. From now, whenever you click only “Save” icon, a spectrum w
43、ill be saved with common file name in the folder numerically. The files will be saved with TXT file format.FolderInput folder name to save spectrum files.Common file nameInput common file name of spectra.Ext.Extension name of spectrum files.2.4. SamplingIf you want to open a “Sampling“ window, click
44、 on the S” icon. I am sorry you cannot find and use it from the pull-down menu. Please use the icon.AUTOIt is an automatic measurement mode. It operates almost similar to the fruits packing house systems. So, it can measure a spectrum of the target, a reference filter and a dark state, automatically
45、. To save all spectra which have a lot of target, reference and offset spectra in a file, you first have to click “Save” icon in the right-hand side. And, click “Save” icon again, after you finish to measure spectra in the AUTO mode. Then, “Save As“ window opens, write your file name that may as wel
46、l write without an extension in the entry box. du have to convert the file, because the file is saved with a binary format. To convert the binary file to TXT format file, drag the file on the “SAM2TXT.exe” file in the program folder. Then, you can find a TXT file of same name as the binary file. Dont forget Fruits-analyzer has to be turned on to operate the PureSpe