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1、Process NarrationProcessInstruction:to direct how to do something step by step Imperative sentence:do+somethingDescription:to explain how to do something step by step Oral:do;you+do;you+can;we+canWritten:third person;passive voice,inanimate subjectHow-to manualCommon practice descriptionOur report？T
2、he polymer solution isAdapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration.Stem oured onto a master made of a silicon substrate and microstructure,and the cured PDMS is then peeled off from
3、 the master.To encapsulate cells with molding,the membrane is immersed in a cell-suspended polymer solution and then removed after solidification of the solution.Cell Research&Therapy 2019,10(1).DOI:10.1186/s13287-018-1130-8Immediately after the central corneal button had been used for corneal trans
4、plantation,the remaining corneoscelral rim was transferred in Optisol-GS and transported at 4 C to the laboratory.The corneoscleral tissue was rinsed three times with PBS and the CEC were isolated by enzymatic digestion.Adapted from:Zhang Z,Niu G,Choi JS,Giegengack M,AtalaA,Soker S.Bioengineered mul
5、tilayered human corneas from discarded human corneal tissue.Biomedical Materials(Bristol,England).2015(10):035012.PMID 26106974 DOI:10.1088/1748-6041/10/3/035012The polymer solution ispoured onto a master made of a silicon substrate and microstructure,and the cured PDMS is then peeled off from the m
6、aster.To encapsulate cells with molding,the membrane is immersed in a cell-suspended polymer solution and then removedafter solidification of the solution.Adapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in t
7、issue regeneration.Stem Cell Research&Therapy 2019,10(1).DOI:10.1186/s13287-018-1130-8Immediately after the central corneal button had been used for corneal transplantation,the remaining corneoscelral rim was transferred in Optisol-GS and transported at 4 C to the laboratory.The corneoscleral tissue
8、 was rinsed three times with PBS and the CEC were isolated by enzymatic digestion.Adapted from:Zhang Z,Niu G,Choi JS,Giegengack M,AtalaA,Soker S.Bioengineered multilayered human corneas from discarded human corneal tissue.Biomedical Materials(Bristol,England).2015(10):035012.PMID 26106974 DOI:10.108
9、8/1748-6041/10/3/035012passive voicepassive voiceThe polymer solution ispoured onto a master made of a silicon substrate and microstructure,and the cured PDMS is then peeled off from the master.To encapsulate cells with molding,the membrane is immersed in a cell-suspended polymer solution and then r
10、emovedafter solidification of the solution.Adapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration.Stem Cell Research&Therapy 2019,10(1).DOI:10.1186/s13287-018-1130-8Immediately after the cent
11、ral corneal button had been used for corneal transplantation,the remaining corneoscelral rim was transferred in Optisol-GS and transported at 4 C to the laboratory.The corneoscleral tissue was rinsed three times with PBS and the CEC were isolated by enzymatic digestion.Adapted from:Zhang Z,Niu G,Cho
12、i JS,Giegengack M,AtalaA,Soker S.Bioengineered multilayered human corneas from discarded human corneal tissue.Biomedical Materials(Bristol,England).2015(10):035012.PMID 26106974 DOI:10.1088/1748-6041/10/3/035012past tensepresent tenseThe polymer solution ispoured onto a master made of a silicon subs
13、trate and microstructure,and the cured PDMS is then peeled off from the master.To encapsulate cells with molding,the membrane is immersed in a cell-suspended polymer solution and then removedafter solidification of the solution.Adapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal ste
14、m cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration.Stem Cell Research&Therapy 2019,10(1).DOI:10.1186/s13287-018-1130-8Immediately after the central corneal button had been used for corneal transplantation,the remaining corneoscelral rim was transferred in Opt
15、isol-GS and transported at 4 C to the laboratory.The corneoscleral tissue was rinsed three times with PBS and the CEC were isolated by enzymatic digestion.Adapted from:Zhang Z,Niu G,Choi JS,Giegengack M,AtalaA,Soker S.Bioengineered multilayered human corneas from discarded human corneal tissue.Biome
16、dical Materials(Bristol,England).2015(10):035012.PMID 26106974 DOI:10.1088/1748-6041/10/3/035012NarrationDescriptionThe polymer solution ispoured onto a master made of a silicon substrate and microstructure,and the cured PDMS is then peeled off from the master.To encapsulate cells with molding,the m
17、embrane is immersed in a cell-suspended polymer solution and then removedafter solidification of the solution.Adapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration.Stem Cell Research&Therapy
18、 2019,10(1).DOI:10.1186/s13287-018-1130-8Using this technology,Yao et al.produced alginate microspheres for rat adipose-derived stem cells(rADSCs).The rADSC-laden microspheres were formed by dropping the cell-suspended alginate solution into a calcium chloride solution.The authors considered four pa
19、rameters such as.Adapted from:Kim H,Bae C,Kook Y-M,Koh W-G,Lee K,Park M.H.Mesenchymal stem cell 3D encapsulation technologies for biomimetic microenvironment in tissue regeneration.Stem Cell Research&Therapy 2019,10(1).DOI:10.1186/s13287-018-1130-8DescriptionNarrationProcessInstruction:to direct how
20、 to do something step by step Imperative sentence:do+somethingDescription:to explain how to do something step by step Oral:do;you+do;you+can;we+canWritten:third person;passive voice,inanimate subjectHow-to manualNarration:to report how something was done step by step Report on a specific studyCommon
21、 practice descriptionpresent tensepast tenseProcess/ProcedureInstructionDescriptionNarrationPurpose Follow the actionsExplain in generalTell what happenedSentenceDo(sth.).(You/We)(can)do(sth.).Sth.is done.Sb.did(sth.)Sth.was doneSubject(you)you/weInanimate subjectwe/theyInanimate subjectTensePresentPastMediaHow-toUser ManualNews reportText bookNews reportExperiment report