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1、blood1医学课件2医学课件Constituents Constituents of bloodof bloodbloodblood plasma plasma （55%55%）bloodblood cell cell （45%45%）EryEryLeuLeuBlood Blood plateletplateletwaterwater（90%90%）othersothers3医学课件(erythrocyte,red blood cell,RBC)(erythrocyte,red blood cell,RBC)staining method：WrightWright or or Giemsa.
2、Giemsa.4医学课件SEM5医学课件6医学课件ErythrothyteErythrothytesizesize：Diameter7.5-8.5 um.Diameter7.5-8.5 um.shapeshape：biconcave circular discbiconcave circular discmorphologymorphology：no nuclei&other no nuclei&other organelles,varieties.organelles,varieties.ABO Antigen:blood group.ABO Antigen:blood group.cont
3、entscontents：haemoglobinhaemoglobin(Hb)(Hb)。amountamount：35-55lakh/mm3 35-55lakh/mm3。7医学课件ReticulocyteReticulocytepremature RBCpremature RBC:left some ribosome in left some ribosome in the cytoplasmthe cytoplasm.Amount:0.5%-1.5%.Amount:0.5%-1.5%.Can synthesize Hb.Can synthesize Hb.8医学课件leukocytes(wh
4、ite blood cell,WBC)WBCgranulocytesgranulocytesgranulocytesgranulocytesAgranularAgranularAgranularAgranularleukocytesleukocytesleukocytesleukocytes neutrophilsneutrophils(60-70%)(60-70%)(60-70%)(60-70%)eosinophilseosinophils(3%)(3%)(3%)(3%)basophilsbasophils(1%)(1%)(1%)(1%)lymphocyteslymphocytes(20-3
5、0%)(20-30%)(20-30%)(20-30%)monocytesmonocytes(5%)(5%)(5%)(5%)Depending on what?Depending on what?9医学课件（LM）Neutrophilic granulocyteNeutrophilic granulocyte amountamountamountamount：60-70%.60-70%.60-70%.60-70%.DiaDiaDiaDia:10-12um.:10-12um.:10-12um.:10-12um.nucleinucleinucleinuclei：variable.variable.v
6、ariable.variable.roddishroddishroddishroddish&several lobes&several lobes&several lobes&several lobes（2-52-52-52-5）.Granules:fine,stain Granules:fine,stain Granules:fine,stain Granules:fine,stain lightly.lightly.lightly.lightly.functionsfunctionsfunctionsfunctions：chemotaxischemotaxischemotaxischemo
7、taxis&phagocytosisphagocytosisphagocytosisphagocytosis.10医学课件（EM）Neutrophilic granulocyteNeutrophilic granulocyteNuclear lobesAzurophilicgranulesspecificgranules11医学课件(LM)(LM)(LM)(LM)Eosinophilic granulocyteEosinophilic granulocyteamountamount：0.5-3%.0.5-3%.Dia:10-15um.Dia:10-15um.nucleinuclei：two-l
8、obes.two-lobes.granulesgranules：large,large,stain brightly stain brightly withwith acid dyes.acid dyes.Functions:chemotaxisFunctions:chemotaxisFunctions:chemotaxisFunctions:chemotaxis&amoebanamoebanamoebanamoeban movement.movement.movement.movement.*inhibit allergic*inhibit allergic*inhibit allergic
9、*inhibit allergic reaction.reaction.reaction.reaction.*kill parasite*kill parasite*kill parasite*kill parasite.12医学课件(EMEMEMEM)Eosinophilic granulocyteEosinophilic granulocytecrystalloid13医学课件(LM)Basophilic granulocyteBasophilic granulocyte amountamountamountamount：0-1%.0-1%.0-1%.0-1%.Dia:10-12 um.D
10、ia:10-12 um.Dia:10-12 um.Dia:10-12 um.nucleinucleinucleinuclei：irregular,shaped irregular,shaped irregular,shaped irregular,shaped“S”“S”“S”“S”.granulesgranulesgranulesgranules：metachromaticalmetachromaticalmetachromaticalmetachromatical,purple-purple-purple-purple-blue,irblue,irblue,irblue,ir-regula
11、r in size®ular in size®ular in size®ular in size&distribution.distribution.distribution.distribution.14医学课件(EM)Basophilic granulocyteBasophilic granulocyte15医学课件（LM）LymphocyteLymphocyteAmountAmountAmountAmount：20-30%.20-30%.20-30%.20-30%.DiaDiaDiaDia:virablevirablevirablevirable.nucleinucle
12、inucleinuclei：largelargelargelarge，spherical,indentatispherical,indentatispherical,indentatispherical,indentation on one on on one on on one on on one side,stain densely.side,stain densely.side,stain densely.side,stain densely.cytoplasmcytoplasmcytoplasmcytoplasm：scantyscantyscantyscanty,clear bluec
13、lear blueclear blueclear blue.ClassificationClassification:TC BC KC NKCTC BC KC NKCTC BC KC NKCTC BC KC NKC 16医学课件（EM）LymphocyteLymphocyte17医学课件（LM）Monocyte Monocyte AmountAmountAmountAmount：5%.5%.5%.5%.sizesizesizesize：the biggest the biggest the biggest the biggest cells in blood.cells in blood.ce
14、lls in blood.cells in blood.，nucleinucleinucleinuclei：kidney,horse-shoekidney,horse-shoekidney,horse-shoekidney,horse-shoe.cytoplasmcytoplasmcytoplasmcytoplasm：abundant,basic,abundant,basic,abundant,basic,abundant,basic,grey blue.grey blue.grey blue.grey blue.Mononuclear Mononuclear Mononuclear Mono
15、nuclear phagocyte system.phagocyte system.phagocyte system.phagocyte system.18医学课件（EM）Monocyte Monocyte Azurophilicgranulesmicrovili19医学课件（LM）Blood plateletBlood platelet Are Are Are Are small masses of small masses of small masses of small masses of cytoplasm shed from cytoplasm shed from cytoplasm
16、 shed from cytoplasm shed from megakaryocytemegakaryocytemegakaryocytemegakaryocyte.Irregular disc,Irregular disc,Irregular disc,Irregular disc,biconvex.No nuclei,In biconvex.No nuclei,In biconvex.No nuclei,In biconvex.No nuclei,In groups.groups.groups.groups.A granular central part.A granular centr
17、al part.A granular central part.A granular central part.A clear outer zone.A clear outer zone.A clear outer zone.A clear outer zone.Functions:blood Functions:blood Functions:blood Functions:blood clotting&coagulation.clotting&coagulation.clotting&coagulation.clotting&coagulation.20医学课件（EM）Blood plateletBlood platelet特殊颗粒特殊颗粒特殊颗粒特殊颗粒Granular partClear area21医学课件22医学课件Review!23医学课件 LM?24医学课件 EMNeutrophil!25医学课件LM Large Horseshoe nucleus26医学课件 LM monocyte！27医学课件 LM28医学课件 EMEosinophil!29医学课件 LM?30医学课件 EMEMLymphocyte!31医学课件