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1、欢迎共阅常见缓冲溶液配制方法乙醇醋酸铵缓冲液(pH3.7)：取 5mol/L 醋酸溶液 15.0ml，加乙醇 60ml 和水20ml，用 10mol/L 氢氧化铵溶液调节pH值至 3.7，用水稀释至 1000ml。三羟甲基氨基甲烷缓冲液(pH8.0)：取三羟甲基氨基甲烷12.14g，加水 800ml，搅拌溶解，并稀释至1000ml，用 6mol/L 盐酸溶液调节 pH值至 8.0。三羟甲基氨基甲烷缓冲液(pH8.1)：取氯化钙 0.294g，加 0.2mol/L 三羟甲基氨基甲烷溶液 40ml 使溶解，用 1mol/L 盐酸溶液调节 pH值至 8.1，加水稀释至 100ml。三羟甲基氨基甲烷缓
2、冲液(pH9.0)：取三羟甲基氨基甲烷6.06g，加盐酸赖氨酸 3.65g，氯化钠 5.8g，乙二胺四醋酸二钠0.37g，再加水溶解使成1000ml，调节pH值至 9.0。乌洛托品缓冲液：取乌洛托品 75g，加水溶解后，加浓氨溶液4.2ml，再用水稀释至 250ml。巴比妥缓冲液(pH7.4)：取巴比妥钠 4.42g，加水使溶解并稀释至400ml，用2mol/L盐酸溶液调节 pH值至 7.4，滤过。巴比妥缓冲液(pH8.6)：取巴比妥 5.52g 与巴比妥钠 30.9g，加水使溶解成2000ml。巴比妥氯化钠缓冲液(pH7.8)：取巴比妥钠 5.05g，加氯化钠 3.7g 及水适量使溶解，另取
3、明胶0.5g 加水适量，加热溶解后并入上述溶液中。然后用0.2mol/L 盐酸溶液调节 pH值至 7.8，再用水稀释至 500ml。甲酸钠缓冲液(pH3.3)：取 2mol/L 甲酸溶液 25ml，加酚酞指示液 1 滴，用 2mol/L氢氧化钠溶液中和，再加入2mol/L 甲酸溶液 75ml,用水稀释至 200ml,调节 pH值至3.253.30。欢迎共阅邻苯二甲酸盐缓冲液(pH5.6)：取邻苯二甲酸氢钾10g，加水 900ml,搅拌使溶解，用氢氧化钠试液(必要时用稀盐酸)调节 pH值至 5.6，加水稀释至 1000ml，混匀。枸橼酸盐缓冲液：取枸橼酸 4.2g,加 1mol/L 的 20乙醇
4、制氢氧化钠溶液40ml使溶解,再用 20乙醇稀释至 100ml。枸橼酸盐缓冲液(pH6.2)：取 2.1枸橼酸水溶液，用 50氢氧化钠溶液调节pH值至 6.2。枸橼酸磷酸氢二钠缓冲液(pH4.0)：甲液：取枸橼酸 21g或无水枸橼酸 19.2g,加水使溶解成 1000ml，置冰箱内保存。乙液：取磷酸氢二钠71.63g，加水使溶解成1000ml。取上述甲液 61.45ml 与乙液 38.55ml 混合，摇匀。氨氯化铵缓冲液(pH8.0)：取氯化铵 1.07g，加水使溶解成 100ml，再加稀氨溶液(130)调节 pH值至 8.0。氨氯化铵缓冲液(pH10.0)：取氯化铵 5.4g，加水 20ml
5、 溶解后，加浓氯溶液35ml，再加水稀释至 100ml。硼砂氯化钙缓冲液(pH8.0)：取硼砂 0.572g 与氯化钙 2.94g，加水约 800ml 溶解后,用 1mol/L 盐酸溶液约 2.5ml 调节 pH值至 8.0，加水稀释至 1000ml。硼砂碳酸钠缓冲液(pH10.8 11.2)：取无水碳酸钠 5.30g,加水使溶解成1000ml；另取硼砂 1.91g，加水使溶解成 100ml。临用前取碳酸钠溶液973ml 与硼砂溶液 27ml，混匀。硼酸氯化钾缓冲液(pH9.0)：取硼酸 3.09g,加 0.1mol/L 氯化钾溶液 500ml使溶解，再加 0.1mol/L 氢氧化钠溶液 21
6、0ml。醋酸盐缓冲液(pH3.5)：取醋酸铵 25g，加水 25ml 溶解后，加 7mol/L 盐酸溶液38ml，用 2mol/L 盐酸溶液或 5mol/L 氨溶液准确调节pH值至 3.5(电位法指示)，用水稀释至 100ml，即得。文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O
7、10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB1
8、0Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O
9、6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE
10、3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T
11、9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编
12、码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A
13、4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3欢迎共阅醋酸锂盐缓冲液(pH3.0)：取冰醋酸 50ml，加水 800ml 混合后，用氢氧化锂调节 pH值至 3.0，再加水稀释至1000ml。醋酸醋酸钠缓冲液(pH3.6)：取醋酸钠 5.1g，加冰醋酸 20ml，再加水稀释至250ml。醋酸醋酸钠缓冲液(pH3.7)：取无水醋酸钠 20g，加水 300ml 溶解后，加溴酚蓝指示液 1ml 及冰醋酸 6080ml，至溶液从蓝色转变为纯绿色，再加水稀释至1000ml。醋酸醋酸钠缓冲液(pH3.8)：取 2mol/L 醋酸钠溶液 13ml 与 2mol/L 醋酸溶液87ml，加每
14、1ml 含铜 1mg的硫酸铜溶液 0.5ml，再加水稀释至1000ml。醋酸醋酸钠缓冲液(pH4.5)：取醋酸钠 18g，加冰醋酸 9.8ml，再加水稀释至1000ml。醋酸醋酸钠缓冲液(pH4.6)：取醋酸钠 5.4g，加水 50ml 使溶解，用冰醋酸调节 pH值至 4.6，再加水稀释至 100ml。醋酸醋酸钠缓冲液(pH6.0)：取醋酸钠 54.6g，加 1mol/L 醋酸溶液 20ml 溶解后，加水稀释至500ml。醋酸醋酸钾缓冲液(pH4.3)：取醋酸钾 14g，加冰醋酸 20.5ml，再加水稀释至1000ml。醋酸醋酸铵缓冲液(pH4.5)：取醋酸铵 7.7g，加水 50ml 溶解后
15、，加冰醋酸 6ml与适量的水使成100ml。醋酸醋酸铵缓冲液(pH6.0)：取醋酸铵 100g，加水 300ml 使溶解，加冰醋酸7ml，摇匀。磷酸三乙胺缓冲液：取磷酸约 4ml与三乙胺约 7ml，加 50甲醇稀释至 1000ml，用磷酸调节 pH值至 3.2。文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文
16、档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV
17、6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F
18、4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 H
19、B10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A
20、2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8
21、ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G
22、6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3欢迎共阅磷酸盐缓冲液：取磷酸二氢钠 38.0g，与磷酸氢二钠 5.04g，加水使成 1000ml，即得。磷酸盐缓冲液(pH2.0)：甲液：取磷酸 16.6ml，加水至 1000ml，摇匀。乙液：取磷酸氢二钠 71.63g，加水使溶解成 1000ml。取上述甲液 72.5ml 与乙液 27.5ml 混合，摇匀。磷酸盐缓冲液(pH2.5)：取磷酸二氢钾 100g，加水 800ml，用盐酸调节 pH至 2.5，用水稀释至 1000ml。磷酸盐缓冲液(pH5.0)：取 0.2mol/L 磷酸二氢钠溶液一定量，
23、用氢氧化钠试液调节 pH值至 5.0。磷酸盐缓冲液(pH5.8)：取磷酸二氢钾 8.34g 与磷酸氢二钾 0.87g,加水使溶解成1000ml。磷酸盐缓冲液(pH6.5)：取磷酸二氢钾 0.68g，加 0.1mol/L 氢氧化钠溶液 15.2ml，用水稀释至 100ml。磷酸盐缓冲液(pH6.6)：取磷酸二氢钠 1.74g、磷酸氢二钠 2.7g 与氯化钠 1.7g，加水使溶解成 400ml。磷酸盐缓冲液(含胰酶)(pH6.8)：取磷酸二氢钾6.8g，加水 500ml使溶解，用0.1mol/L 氢氧化钠溶液调节pH值至 6.8；另取胰酶 10g，加水适量使溶解，将两液混合后,加水稀释至 1000
24、ml。磷酸盐缓冲液(pH6.8)：取 0.2mol/L 磷酸二氢钾溶液 250ml，加 0.2mol/L 氢氧化钠溶液 118ml，用水稀释至 1000ml，摇匀。磷酸盐缓冲液(pH7.0)：取磷酸二氢钾 0.68g，加 0.1mol/L 氢氧化钠溶液 29.1ml，用水稀释至 100ml。磷酸盐缓冲液(pH7.2)：取 0.2mol/L 磷酸二氢钾溶液 50ml 与 0.2mol/L 氢氧化文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10
25、HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3
26、A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8
27、 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5
28、G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3
29、文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:C
30、V6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7
31、F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3欢迎共阅钠溶液 35ml，加新沸过的冷水稀释至200ml，摇匀。磷酸盐缓冲液(pH7.3)：取磷酸氢二钠 1.9734g 与磷酸二氢钾 0.2245g,加水使溶解成 1000ml，调节 pH值至 7.3。磷酸盐缓冲液(pH7.4)：取磷酸二氢钾 1.36g，加 0.1mol/L 氢氧化钠溶液 79ml，用水稀释至 200ml。磷酸盐缓冲液(pH7.6)：取磷酸二氢钾 2
32、7.22g，加水使溶解成 1000ml，取 50ml，加 0.2mol/L 氢氧化钠溶液 42.4ml，再加水稀释至200ml。磷酸盐缓冲液(pH7.8)：甲液：取磷酸氢二钠 35.9g，加水溶解，并稀释至 500ml。乙液：取磷酸二氢钠2.76g，加水溶解，并稀释至100ml。取上述甲液 91.5ml 与乙液 8.5ml 混合，摇匀。磷酸盐缓冲液(pH7.8 8.0)：取磷酸氢二钾 5.59g 与磷酸二氢钾 0.41g，加水使溶解成 1000ml，即得。1.甘氨酸-盐酸缓冲液（0.05M）：X ml 0.2M 甘氨酸+Y ml 0.2M 盐酸 再加水稀释至200ml pH X/ml Y/ml
33、 pH X/ml Y/ml 2.2 50 44.0 3.0 50 11.4 2.4 50 32.4 3.2 50 8.2 2.6 50 24.2 3.4 50 6.4 2.8 50 16.8 3.6 50 5.0 甘氨酸分子量 75.07 0.2M甘氨酸溶液含 15.01g/L 2.邻苯二甲酸-盐酸缓冲液（0.05M）：X ml 0.2M 邻苯二甲酸氢钾 +Y ml 0.2M盐酸 再加水稀释至 20ml pH X/ml Y/ml pH X/ml Y/ml 2.2 5 4.670 3.2 5 1.470 2.4 5 3.960 3.4 5 0.990 2.6 5 3.295 3.6 5 0.59
34、7 2.8 5 2.642 3.8 5 0.263 文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10
35、Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6
36、M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3
37、Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9
38、D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码
39、:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4
40、S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3欢迎共阅3.0 5 2.032 0.2M 邻苯二甲酸氢钾溶液含40.85g/L 3.磷酸氢二钠-柠檬酸缓冲液：X m
41、l 0.2M Na2HPO4+Yml 0.1M 柠檬酸pH X/ml Y/ml pH X/ml Y/ml pH X/ml Y/ml 2.2 0.40 19.6 4.2 8.2 11.72 6.2 13.22 6.78 2.4 1.24 18.76 4.4 8.82 11.18 6.4 13.85 6.15 2.6 2.18 17.82 4.6 9.35 10.65 6.6 14.55 5.45 2.8 3.17 16.83 4.8 9.86 10.14 6.8 15.45 4.55 3.0 4.11 15.89 5.0 10.30 9.70 7.0 16.47 3.53 3.2 4.94 15
42、.06 5.2 10.72 9.28 7.2 17.39 2.61 3.4 5.70 14.30 5.4 11.15 8.85 7.4 18.17 1.83 3.6 6.44 13.56 5.6 11.60 8.40 7.6 18.73 1.27 3.8 7.10 12.90 5.8 12.09 7.91 7.8 19.15 0.85 4.0 7.71 12.29 6.0 12.63 7.37 8.0 19.45 0.55 Na2HPO4分子量 141.98 0.2M溶液含 28.40g/L Na2HPO42H2O分子量 178.05 0.2M溶液含 35.61g/L C6H8O7H2O分子量
43、 210.14 0.1M溶液含 21.01g/L 4.柠檬酸-氢氧化钠-盐酸缓冲液（先不配）pH 钠离子浓度/M 柠檬酸 C6H8O7H2O/g 氢氧化钠NaOH/g 浓盐酸 HCl/ml 终体积/L 2.2 0.20 210 84 160 10 3.1 0.20 210 83 116 10 3.3 0.20 210 83 106 10 4.3 0.20 210 83 45 10 5.3 0.35 245 144 58 10 5.8 0.45 285 186 105 10 6.5 0.38 266 156 126 10 使用时可以每升中加入1g 酚，若最后 pH有变化，再用少量 50%氢氧化钠
44、溶液或浓盐酸调节，冰箱保存。5.柠檬酸-柠檬酸钠缓冲液（0.1M）pH 0.1M 柠檬酸/ml 0.1 mol/L柠檬酸钠/mL pH 0.1M 柠檬酸/ml 柠檬酸 C6H8O7H2O/g 3.0 18.6 1.4 5.0 8.2 11.8 3.2 17.2 2.8 5.2 7.3 12.7 3.4 16.0 4.0 5.4 6.4 13.6 文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3
45、文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:C
46、V6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7
47、F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10
48、HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3
49、A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8
50、 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5G6T9D3文档编码:CV6A4S7F4O10 HB10Q3A2O6M8 ZE3Q5