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1、鱼鸭混养池塘罗非鱼无乳链球菌病的病原分离鉴定及其防治打开文本图片集【摘要】：p ：【目的】查明罗非鱼与鸭混养模式下引起罗非鱼发病的主要病原，并探索微生物与中药综合防治的有效方法，为建立安全、高效的鱼鸭混养模式提供科学依据。【方法】从广西南宁市隆安县那桐镇某罗非鱼与鸭混养池塘的发病罗非鱼中取样进行病原菌分离纯化，对获得的纯化分离菌株进行16S rRNA序列测序及人工感染试验和药敏试验，并在药敏试验的基础上采用内服结合外用消毒的方式进行治疗，且在罗非鱼痊愈后使用微生物制剂（成分为芽孢杆菌、乳酸菌、酵母菌和光合菌等）调节池塘水质，同时给罗非鱼投喂微生物制剂和中药（三黄散、肝胆利康散和黄芪多糖）。【结
2、果】引起罗非鱼与鸭混养池塘中罗非鱼发病的病原菌为无乳链球菌（Streptococcus agalactiae），其对罗非鱼的半数致死浓度（LD50）为4.7106 CFU/mL，对罗红霉素、青霉素、氯霉素、磺胺异恶唑、阿莫西林、利福平、头孢克洛、呋喃唑酮、氟本尼考、头孢氨苄和头孢派酮等抗菌药物高度敏感，对诺氟沙星、庆大霉素和卡那霉素不敏感（耐药）。通过内服氟苯尼考、三黄散、维生素K3和黄芪多糖并全塘泼洒戊二酫与苯扎溴铵混合液及过硫酸氢钾，连续用药14 d后罗非鱼病情基本得到控制。定期泼洒微生物制剂，结合罗非鱼内服微生物制剂和中药的效果良好，即使在高温季节也未出现水质恶化和鱼类发病现象。【结论】
3、引起鱼鸭混养池塘罗非鱼发病的病原菌为无乳链球菌，可采取氟本尼考与三黄散、维生素K3和黄芪多糖联合用药的方法进行治疗。生产上，在鱼鴨混养池塘泼洒微生物制剂及罗非鱼内服微生物制剂和中药，对改善池塘水质及预防疾病发生具有积极作用，能有效降低鱼鸭混养模式下鱼类的发病率。【关键词】：p ： 罗非鱼;鱼鸭混养模式;无乳链球菌;微生物制剂;中药中图分类号： S943.125.42 文献标志码： A 文章编号：2095-1191（20_）11-2583-09Isolation， identification and prevention of the pathogen of Streptococcus aga
4、lactiae disease in tilapia of fish-duck polyculture pondCHEN Fu-yan1， LI Jian-bin1， OUYANG _ian-hua2， YANG _ue-ming1， HUANG Cai-lin3， LI Da-lie1， LEI Ai-ying1， LIANG Wan-wen1_（1Guang_i Academy of Fisheries Sciences/Guang_i Key Laboratory of Aquatic Geic Breeding and Healthy Aquaculture， Nanning 5300
5、21， China; 2Guang_i Fangcao Angui Biotechnology Co.， Ltd.， Nanning530001， China; 3Guang_i Aquatic Products Breeding Center， Nanning 530031， China）Abstract：【Objective】To identify the pathogens of tilapia diseases in a tilapia-duck polyculture pond， e_plore the ways to prehensively control diseases by
6、 microorganisms and Chinese herbal medicines， and provide scientific basis for establishing a safe and efficient fish-duck polyculture model.【Method】The pathogen of tilapia fish disease in a tilapia-duck polyculture pond in Natong， Longan， Nanning were isolated and identified， and 16S rRNA sequencin
7、g， artificial infection test and drug sensitivity test on the purified isolate were performed.Then oral administration bined with e_ternal disinfection was used for treatment based on the drug sensitivity test.After the tilapia were cured， the microbial preparations（the ingredients were bacillus， la
8、ctobacillus， yeast and photosynthetic bacteria， etc.） were used to control the water quality of the pond， and tilapia were fed with microbial preparations and traditional Chinese medicines（Sanhuangsan， Gandanlikangsan and Astragalus polysaccharides）.【Result】The pathogen of the tilapia disease in thi
9、s pond was identified as Streptococcus agalactiae， and its medium lethal concentration（LD50） for tilapia was 4.7106 CFU/mL.It was highly sensitive to ro_ithromycin， penicillin， chloromycetin， sulfaniso_azole， amo_icillin， rificin， cefaclor， furazolidone， flubenicol， cefale_in and cefoperazone， and r
10、esistant to norflo_acin， gentamicin and kanamycin.After continuous oral administration of flufenicol， Sanhuangsan， vitamin K3， astragalus polysaccharides， and the whole pond sprayed with a concentrated glutaridine/benzamium bromide solution and potassium persulfate for 14 d， tilapia disease was basi
11、cally controlled.The spray of microbial preparations regularly bined with oral administration of microbial preparations and Chinese herbal medicines had sound effects on water quality， no deterioration of water quality and disease occurred even in hot seasons.【Conclusion】The pathogen causing tilapia
12、 in fish-duck polyculture ponds was S.agalactiae， which can be treated with the bination of flubenico， Sanhuangsan， vitamin K3 and astragalus polysaccharide.In production， spraying microbial preparations and oral administration of microbial preparations and Chinese herbal medicines on fish-duck polyculture ponds has a positive effect on improving water quality and preventing disease occurrence， and can effectively reduce the disease incidence of fish in polyculture modes.第 3 页 共 3 页