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1、-Hoagland-营养液配方(试验总结)-第 3 页Hoagland 营养液配方， 荷格伦特营养液的配方如下: 1.大量元素 每升培养液中加入克数或摩尔数盐类浓度g/Lmol/Lg/15 LCa(NO3)24H2O1.180.00517.7KNO30.510.0057.65MgSO47H2O0.490.0027.35KH2PO40.140.0012.12.微量元素 每升培养液中加入的毫克数盐类浓度（mg/L）mg/15 LH3BO32.8642.9MnCl24H2O1.8127.15ZnSO47H2O0.223.3CuSO45H2O0.081.2H2MoO4H2O/Na2MoO42H2O0.
2、02/0.030.483.每升培养液中加入2 mL FeEDTA溶液(即乙二胺四乙酸铁盐溶液)。铁盐溶液：七水硫酸亚铁 2.78g 乙二胺四乙酸二钠（EDTA-Na2）3.73g 蒸馏水 500 mL 配制500 mL的铁盐溶液：1. 取两只洁净干燥烧杯，分别加入3.73 g EDTA-Na2和2.78 g FeSO47H2O固体。2. 取1 L纯水煮沸，冷却至70后，量取300 mL，加入装有EDTA-Na2的烧杯中，进行搅拌溶解。3. 量取200 mL纯水，冷却后加入另一烧杯中，搅拌溶解。4. 然后将FeSO47H2O溶液缓缓加入到EDTA-Na2溶液中，边加边搅拌。5. 最后，将铁盐溶液
3、倒入棕色瓶中（黑暗），低温保存。通常应当先配出各种盐类的浓缩液。注意避免浓缩液中出现沉淀。使用时按一定的比例加水稀释到要求的浓度。采用循环供液时，营养液中的矿质元素被植物体吸收后，应当及时进行调节，使营养液仍旧符合原配方的要求。 以下 是老外的 有些看不懂 呵呵Recipe for Hoaglands Complete Nutrient Solution This is made essentially according to the following reference: D.R. Hoagland and D.I. Arnon. The water-culture method of
4、growing plants without soil. Calif. Agr. Expt. Sta. Circ. 347. 1950. There is one change and that is in the form of iron added. Prepare the following stock solutions (1-6) and use the amounts indicated to prepare 1 liter ( final volume ) of nutrient solution: 1. 1.00 M NH4H2PO4 use 1 mL/L of nutrien
5、t solution 2. 1.00 M KNO3 use 6 mL/L of nutrient solution 3. 1.00 M Ca(NO3 )2 use 4 mL/L of nutrient solution 4. 1.00 M MgSO4 use 2 mL/L of nutrient solution Micronutrient stocks: combine the following amount of salts in a total volume of one liter of water, and then use 1 mL/L of this entire stock
6、mixture (5) along with the stocks above (1-4) and the iron stock (6) described below to make up a total of 1 L of nutrient solution. 5. 2.86 gm H3BO3 1.81 gm MnCl2 .4H2O 0.22 gm ZnSO4 .7H2 O 0.08 gm CuSO4 . 5H2O 0.02 gm H2MoO4 . H2O (Assaying 85% MoO3) 6. Iron stock: to the above 5 stocks add 0.25 m
7、l of this iron stock for 1 liter of nutrient solution. To make up the iron stock, take 26.1 g EDTA and dissolve in 286 ml water that has 19 g KOH . Then dissolve 24.9 FeSO4.7H2O in 500 ml water. Slowly add the iron sulfate solution to the potassium EDTA solution and aerate this solution overnight wi
8、th stirring. The pH rises to about 7.1 and the solution is wine red and very little precipitation occurs. Make to 1 liter final volume and store in a bottle covered with foil (dark). Note: Hoaglands recipe called for 1 ml of 0.5% iron tartrate stock per liter of nutrient solution but we use the above substitution.