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1、限制性内切酶兼容粘性末端 Compatible Cohesive Ends matinlda Enzyme Ligated To Recleaved By Acc65I(G/GTACC) BanI (G/GTACC) Acc65I, BanI, KpnI, NlaIV, RsaI BsiWI, BsrGI RsaI AccI(GT/CGAC) AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI - (GT/CGAC) ClaI, BstBI, TaqI TaqI AciI(C/CGC) AccI (GT/CGAC), AclI, ClaI, Bst
2、BI, TaqI - BsaHI (GR/CGCC), HinP1I, NarI AciI HpaII HpaII AclI(AA/CGTT) AccI (GT/CGAC), AciI, ClaI, BstBI, HinP1I, HpaII, NarI, TaqI - AgeI(A/CCGGT) AvaI (C/CCGGG), XmaI HpaII, NciI, ScrFI BsaWI, BspEI BsaWI, HpaII BsrFI (A/CCGGT), SgrAI (CA/CCGGTG) AgeI, BsaWI, BsrFI, HpaII NgoMIV BsrFI, HpaII ApaI
3、 (GGGCC/C) BanII (GGGCC/C), Bsp1286I (GGGCC/C) ApaI, BanII, Bsp120I, Bsp1286I, HaeIII, NlaIV, Sau96I ApaLI (G/TGCAC) SfcI (C/TGCAG) BsgI ApoI(A/AATTY) EcoRI ApoI, Tsp509I (G/AATTY) EcoRI ApoI, EcoRI, Tsp509I (R/AATTY) MfeI, Tsp509I Tsp509I AscI (GG/CGCGCC) AflIII (A/CGCGT), MluI BstUI, HhaI BssHII B
4、ssHII, BstUI, Cac8I, HhaI AseI(AT/TAAT) BfaI, Csp6I, NdeI - MseI MseI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 1 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By AsiSI(GCGAT/CGC) BsiEI (CGAT/CG) DpnII, PvuI PacI MseI PvuI DpnII, PvuI AvaI(C/CCGGG) AgeI,
5、 BsaWI, BspEI, BsrFI (R/CCGGY), NgoMIV, SgrAI (CR/CCGGYG) HpaII, NciI, ScrFI (C/TCGAG) XhoI AvaI, TaqI, XhoI (C/TCGAG) SalI TaqI (C/CCGGG) XmaI AvaI, BsaJI, HpaII, NciI, ScrFI, SmaI AvaII (G/GWCC) PpuMI (RG/GACCY) AvaII, NlaIV, Sau96I RsrII AvaII, Sau96I PpuMI (RG/GTCCY) AvaII, BsmFI, NlaIV, Sau96I
6、AvrII (C/CTAGG) NheI, SpeI, XbaI BfaI StyI (C/CTAGG) AvrII, BfaI, BsaJI, StyI BamHI (G/GATCC) BclI, DpnII AlwI, DpnII BglII, BstYI (R/GATCY) AlwI, BstYI, DpnII BstYI (G/GATCC) AlwI, BamHI, BstYI, DpnII, NlaIV BanI(G/GTACC) Acc65I Acc65I, BanI, KpnI, NlaIV, RsaI (G/GCGCC) KasI BanI, BsaHI, HaeII, Hha
7、I, KasI, NarI, NlaIV (G/GTACC) BsiWI, BsrGI RsaI BanII(GGGCC/C) ApaI, Bsp1286I (GGGCC/C) ApaI, BanII, Bsp1286I, HaeIII, NlaIV, Sau96I (GAGCT/C) Bsp1286I (GAGCT/C), SacI AluI, BanII, BsiHKAI, Bsp1286I, SacI BclI(T/GATCA) BamHI, BstYI (R/GATCY) AlwI, DpnII BglII, MboII DpnII 名师资料总结 - - -精品资料欢迎下载 - - -
8、 - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 2 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By BfaI(C/TAG) AseI, Csp6I, MseI, NdeI - BglII(A/GATCT) BamHI, BstYI (R/GATCY) AlwI, BstYI, DpnII BclI, DpnII DpnII BsaHI(GR/CGYC) AccI (GT/CGAC), ClaI, BstBI, TaqI - (GA/CGYC) AciI, HinP1I
9、HgaI (GG/CGYC) AciI, HinP1I HhaI (GG/CGYC) HpaII AciI (GA/CGYC) NarI BsaHI, HgaI (GG/CGYC) NarI BanI, BsaHI, HaeII, HhaI, NarI, NlaIV BsaWI (W/CCGGW) AgeI, BsrFI (R/CCGGY), SgrAI (CR/CCGGYG) AgeI, BsaWI, BsrFI, HpaII AvaI (C/CCGGG), XmaI HpaII, NciI, ScrFI BspEI BsaWI, BspEI, HpaII BsrFI (R/CCGGY),
10、NgoMIV BsrFI, HpaII NgoMIV HpaII BsiEI(CGAT/CG) PacI MseI (CGAT/CG) PvuI BsiEI, DpnII, PvuI (CGGC/CG) SacII AciI BsiHKAI(GTGCA/C) Bsp1286I (GTGCA/C) BsiHKAI, Bsp1286I Bsp1286I (GAGCA/C) BsiHKAI, Bsp1286I Bsp1286I (GAGCT/C), SacI AluI, BanII, BsiHKAI, Bsp1286I, SacI NsiI - PstI, SbfI BsgI BsiWI (C/GT
11、ACG) Acc65I, BanI (G/GTACC), BsrGI RsaI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 3 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By Bsp1286I(GGGCC/C) ApaI, BanII (GGGCC/C) ApaI, BanII, Bsp1286I, HaeIII, NlaIV, Sau96I (GTGCA/C) BsiHKAI ApaLI, BsiHKAI, Bs
12、p1286I (GGGCC/C) BanII (GGGCC/C) BanII, Bsp1286I (GAGCT/C) BanII (GAGCT/C), BsiHKAI, SacI AluI, BanII, BsiHKAI, Bsp1286I, SacI (GWGCW/C) BsiHKAI BsiHKAI, Bsp1286I (GTGCA/C) NsiI - (GTGCA/C) PstI, SbfI BsgI BspEI (T/CCGGA) AgeI, BsaWI, BsrFI (R/CCGGY), SgrAI (CR/CCGGYG) BsaWI, HpaII AvaI (C/CCGGG), X
13、maI HpaII, NciI, ScrFI BsaWI BsaWI, BspEI, HpaII BsrFI (R/CCGGY), NgoMIV HpaII BspHI (T/CATGA) FatI, NcoI, PciI FatI, NlaIII BsrFI(A/CCGGY) AgeI, BsaWI AgeI, BsaWI, BsrFI, HpaII (G/CCGGY) AgeI, BsaWI, NgoMIV BsrFI, HpaII (R/CCGGY) AvaI (C/CCGGG), XmaI HpaII, NciI, ScrFI (A/CCGGY) BsaWI, BspEI BsaWI,
14、 HpaII (R/CCGGY) BsaWI, BspEI HpaII (G/CCGGY) NgoMIV BsrFI, Cac8I, HpaII, NaeI (CR/CCGGYG) SgrAI BsrFI, HpaII BsrGI (T/GTACA) Acc65I, BanI (G/GTACC), BsiWI RsaI BssHII(G/CGCGC) MluI BstUI, HhaI AscI BssHII, BstUI, Cac8I, HhaI BstBIAccI (GT/CGAC), ClaI, TaqI TaqI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - -
15、- - - - - - - - - - 名师精心整理 - - - - - - - 第 4 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By (TT/CGAA) AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI - BstYI(A/GATCY) BamHI, BglII AlwI, BstYI, DpnII (G/GATCY) BamHI AlwI, BamHI, BstYI, DpnII, NlaIV (R/GATCY) BclI, DpnII DpnII (G/GATCY) Bcl
16、I, DpnII AlwI, DpnII (A/GATCY) BglII BglII, BstYI, DpnII ClaI (AT/CGAT) AccI (GT/CGAC), BstBI, TaqI TaqI AciI, AclI, BsaHI (GR/CGYC), HinP1I, HpaII, NarI - DpnII/MboI/ Sau3AI (/GATC) BamHI, BstYI (R/GATCC) AlwI, DpnII BclI, BglII, BstYI (R/GATCY) DpnII EaeI(Y/GGCCR) PspOMI HaeIII, Sau96I (C/GGCCR) E
17、agI BsiEI, EaeI, EagI, HaeIII (T/GGCCR) EagI EaeI, HaeIII (C/GGCCR) NotI AciI, BsiEI, EaeI, EagI, Fnu4HI, HaeIII (T/GGCCR) NotI AciI, EaeI, Fnu4HI, HaeIII EagI(C/GGCCG) PspOMI HaeIII, Sau96I EaeI (Y/GGCCR) EaeI, HaeIII EaeI (C/GGCCG) BsiEI, EaeI, EagI, HaeIII NotI AciI, BsiEI, EaeI, EagI, Fnu4HI, Ha
18、eIII EcoRI(G/AATTC) ApoI (G/AATTC) ApoI, EcoRI, Tsp509I ApoI (R/AATTY) ApoI, Tsp509I MfeI, Tsp509I Tsp509I FatI(/CATG) BspHI, NcoI, PciI FatI, NlaIII 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 5 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By HinP1I(G/CG
19、C) AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI - AciI, BsaHI (GR/CGCC), NarI HhaI BsaHI (GR/CGTC) HgaI HpaII AciI HpaII/MspI(C/CGG) AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI - AciI, BsaHI (GR/CGCC), HinP1I, NarI AciI KasI(G/GCGCC) BanI (G/GCGCC) BanI, BsaHI, HaeII, HhaI, KasI, NarI, NlaIV MfeI(C/AATTG) Ap
20、oI (R/ATTTY), EcoRI, Tsp509I Tsp509I MluI (A/CGCGT) AscI, BssHII BstUI, HhaI MseI (T/TAA) AseI MseI BfaI, Csp6I, NdeI - NarI (GG/CGCC) AccI (GT/CGAC), AclI, ClaI, BstBI, TaqI - AciI, HinP1I HhaI BsaHI (GR/CGCC) BanI, BsaHI, HaeII, HhaI, NarI, NlaIV BsaHI (GR/CGTC) BsaHI, HgaI HpaII AciI NcoI (C/CATG
21、G) BspHI, FatI, PciI FatI, NlaIII NdeI(CA/TATG) AseI, BfaI, Csp6I, MseI - NgoMIV(G/CCGGC) AgeI, BsaWI, BsrFI (R/CCGGY), SgrAI BsrFI, HpaII AvaI (C/CCGGG), XmaI HpaII, NciI, ScrFI BsaWI, BspEI HpaII BsrFI (R/CCGGC), SgrAI BsrFI, Cac8I, HpaII, NaeI NheIAvrII, SpeI, StyI (C/CTAGG), XbaI BfaI 名师资料总结 - -
22、 -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 6 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By (G/CTAGC) NlaIII(CATG/) SphI, NspI NlaIII NotI(GC/GGCCGC) PspOMI AciI, EaeI, Fnu4HI, HaeIII EagI AciI, BsiEI, EaeI, EagI, Fnu4HI, HaeIII EaeI (Y/GGCCR) AciI, BsiEI, EaeI,
23、Fnu4HI, HaeIII NsiI(ATGCA/T) BsiHKAI (GTGCA/C), Bsp1286I (GTGCA/C), PstI, SbfI - NspI(RCATG/Y) NlaIII, SphI NlaIII PacI (TTAAT/TAA) BsiEI (CGAT/CG), PvuI MseI PciI(A/CATGT) BspHI, FatI, NcoI FatI, NlaIII PpuMI(RG/GWCCY) AvaII, RsrII AvaII, Sau96I (GG/GTCCY) AvaII, RsrII AvaII, BsmFI, NlaIV, Sau96I (
24、GG/GACCY) AvaII, RsrII AvaII, NlaIV, Sau96I PspOMI(G/GGCCC) EaeI (Y/GGCCR), EagI HaeIII, Sau96I NotI AciI, Fnu4HI, HaeIII, Sau96I PspXI (VC/TCGAGB) XhoI, TliI XhoI, TliI SalI TaqI PstI(CTGCA/G) BsiHKAI, Bsp1286I (GTGCA/C) BsgI NsiI - SbfI PstI PvuI(CGAT/CG) AsiSI DpnI, PvuI PacI MseI BsiEI (CGAT/CG)
25、 BsiEI, DpnII, PvuI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 7 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By RsrII(CG/GWCCG) AvaII, PpuMI (RG/GACCY) AvaII, Sau96I PpuMI (RG/GACCY) AvaII, NlaIV, Sau96I PpuMI (RG/GTCCY) AvaII, BsmFI, NlaIV, Sau96I SacI
26、(GAGCT/C) BanII (GAGCT/C), BsiHKAI, Bsp1286I (GAGCT/C) AluI, BanII, BsiHKAI, Bsp1286I, SacI SacII(CCGC/GG) BsiEI (CGGC/CG) AciI SalI(G/TCGAC) PspXI, XhoI TaqI SbfI(CCTGCA/GG) BsiHKAI, Bsp1286I (GTGCA/C) BsgI NsiI - PstI PstI SfcI(C/TGCAG) ApaLI BsgI SgrAI(CR/CCGGYG) See BsrFISpeI(A/CTAGT) AvrII, Nhe
27、I, StyI (C/CTAGG), XbaI BfaI SphI(GCATG/C) NlaIII, NspI NlaIII StyIAvrII AvrII, BfaI, BsaJI, StyI (C/CTAGG) NheI, SpeI, XbaI BfaI (C/CATGG) BspHI NlaIII NcoI BsaJI, NcoI, NlaIII, StyI TaqI(T/CGA) AccI (GT/CGAC), ClaI, BstBI TaqI AciI, AclI, BsaHI (GR/CGYC), HinP1I,HpaII, NarI - Tsp509I(/AATT) ApoI (
28、R/AATTY), EcoRI, MfeI Tsp509I XbaI(T/CTAGA) AvrII, NheI, SpeI, StyI (C/CTAGG) BfaI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 8 页，共 9 页 - - - - - - - - - Enzyme Ligated To Recleaved By XhoI/TliI(C/TCGAG) PspXI XhoI, TliI SalI TaqI XmaI(C/CCGGG) AgeI, BsaWI, BspEI, BsrFI, NgoMIV, SgrAI HpaII, NciI, ScrFI AvaI (C/CCGGG) AvaI, BsaJI, HpaII, NciI, ScrFI, SmaI, XmaI 名师资料总结 - - -精品资料欢迎下载 - - - - - - - - - - - - - - - - - - 名师精心整理 - - - - - - - 第 9 页，共 9 页 - - - - - - - - -