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1、精选优质文档-倾情为你奉上荧光素名称激发波长 (nm)发射波长 (nm)备注1,5 IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-dichlorofluorescein5045295-Carboxyfluorescein (5-FAM)4925185-Carboxynapthofluorescein(pH 10)512/598563/668Ratio Dye, pH5-Carboxytetramethylrhodamine (5-TAMRA)5425685-FAM (5-Carboxyfluorescein
2、)4925185-HAT (Hydroxy Tryptamine)370-415520-5405-Hydroxy Tryptamine (HAT)370-415520-5405-ROX (carboxy-X-rhodamine)5785676045915-TAMRA (5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine 6G5185436-CR 6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-Aminoactinomycin D (7-AAD)5466477-Hydrox
3、y-4-methylcoumarin360449, 4559-Amino-6-chloro-2-methoxyacridine412, 430471, 474ABQ344445Acid Fuchsin540630ACMA (9-Amino-6-chloro-2-methoxyacridine)412, 430471, 474Acridine Orange + DNA502526Acridine Orange + RNA460650Acridine Orange, both DNA & RNA440-480520-650Acridine Red455-600560-680Acridine Yel
4、low470550Acriflavin436520Acriflavin Feulgen SITSA355-425460Aequorin (Photoprotein)466PhotoproteinAFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFPAlexa Fluor 350346342442441Alexa Fluor 430431540Alexa Fluor 488495, 492519, 520Alexa Fluor 532531, 532553, 554Alexa Fluor 546556
5、, 557572, 573Alexa Fluor 568577, 578603Alexa Fluor 594590, 594617, 618Alexa Fluor 633632650Alexa Fluor 647647666Alexa Fluor 660668698Alexa Fluor 680679702Alizarin Complexon530-560, 580580, 624-645Alizarin Red530-560580Allophycocyanin (APC)630, 645655, 660AMC, AMCA-S345445AMCA (Aminomethylcoumarin)34
6、5347425444AMCA-X353442Aminoactinomycin D555655Aminocoumarin346350442445Aminomethylcoumarin (AMCA)345347425444Anilin Blue600Anthrocyl stearate360-381446APC (Allophycocyanin)630, 645655, 660APC-Cy7625-650755APTRA-BTC = Ratio Dye, Zn2+466/380520/530Ratio Dye, Zn2+APTS424505Astrazon Brilliant Red 4G5005
7、85Astrazon Orange R470540Astrazon Red 6B520595Astrazon Yellow 7 GLL450480Atabrine436490ATTO-TAG CBQCA465560ATTO-TAG FQ486591Auramine460550Aurophosphine G450580Aurophosphine450-490515BAO 9 (Bisaminophenyloxadiazole)365395BCECF (high pH)492, 503520,528BCECF (low pH)482520Berberine Sulphate430550Beta L
8、actamase409447, 520BFP blue shifted GFP (Y66H)Blue Fluorescent Protein381, 382, 383445, 447, 448blue shifted GFP (Y66H)Blue Fluorescent ProteinBFP/GFP FRETBimane398490Bisbenzamide360461Bisbenzimide (Hoechst)360461bis-BTC = Ratio Dye, Zn2+455/405529/505Ratio Dye, Zn2+Blancophor FFG390470Blancophor SV
9、370435BOBO -1462481BOBO -3570602Bodipy 492/515490515Bodipy 493/503533549Bodipy 500/510509515Bodipy 505/515502510Bodipy 530/550528547Bodipy 542/563543563Bodipy 558/568558569Bodipy 564/570564570Bodipy 576/589579590Bodipy 581/591584592Bodipy 630/650-X625642Bodipy 650/665-X647665Bodipy 665/676605676Bodi
10、py Fl504, 505511, 513Bodipy FL ATP505514Bodipy Fl-Ceramide505511Bodipy R6G SE528547Bodipy TMR542574Bodipy TMR-X conjugate544573Bodipy TMR-X, SE544570Bodipy TR589617Bodipy TR ATP591620Bodipy TR-X SE588616BO-PRO -1462481BO-PRO -3544570Brilliant Sulphoflavin FF430520BTC - Ratio Dye Ca2+464/401533/529Ra
11、tio Dye Ca2+BTC-5N - atio Dye, Zn2+459/417517/532Ratio Dye, Zn2+Calcein494517Calcein Blue373440Calcium Crimson588, 589611, 615Calcium Green501, 506531Calcium Green-1 Ca2+ Dye506531Ca2+ DyeCalcium Green-2 Ca2+506/503536Ca2+Calcium Green-5N Ca2+506532Ca2+Calcium Green-C18 Ca2+509530Ca2+Calcium Orange5
12、49575576Calcofluor White385, 395, 405437, 440, 445Carboxy-X-rhodamine (5-ROX)576601Cascade Blue377398399420423Cascade Yellow399400550552Catecholamine410470CCF2 (GeneBlazer)CFDA494520CFP - Cyan Fluorescent Protein430, 433, 436, (453)474, 475, 476, (501)Cyan Fluorescent ProteinCFP/YFP FRETChlorophyll4
13、80650Chromomycin A436-460470Chromomycin A445575CL-NERF (Ratio Dye, pH)504/514540Ratio Dye, pHCMFDA494520Coelenterazine Ca2+ Dye, bioluminescence(429)465Ca2+ Dye, bioluminescence,native moleculeCoelenterazine cp (Ca2+ Dye,)(430)442Ca2+ Dye, bioluminescenceCoelenterazine f(437)473Ca2+ Dye, bioluminesc
14、enceCoelenterazine fcp452Ca2+ Dye, bioluminescenceCoelenterazine h(437)464Ca2+ Dye, bioluminescenceCoelenterazine hcp(433)444Ca2+ Dye, bioluminescenceCoelenterazine ip441Ca2+ Dye, bioluminescenceCoelenterazine n(431)467Ca2+ Dye, bioluminescenceCoelenterazine O460575Coumarin Phalloidin387470C-phycocy
15、anineCPM Methylcoumarin384469MethylcoumarinCTC400-450602CTC FormazanCy2489506Cy3.1 8554568Cy3.5581598Cy3514552554566570Cy5.1 8649666Cy5.5675695Cy5649666670Cy7710, 743767, 805Cyan GFP433 (453)475 (501)cyclic AMP Fluorosensor (FiCRhR)500517CyQuant Cell Proliferation Assay480520Cell Proliferation Assay
16、Dabcyl453Dansyl340578Dansyl Amine337517Dansyl Cadaverine335518Dansyl Chloride372518Dansyl DHPE336517Dansyl fluoride356noneDAPI359461Dapoxyl403580Dapoxyl 2374574Dapoxyl 3373574DCFDA504529DCFH (Dichlorodihydrofluorescein Diacetate)505535DDAO463607DHR (Dihydorhodamine 123)505534Di-4-ANEPPS496705Di-8-AN
17、EPPS (non-ratio)488498605713DiA (4-Di-16-ASP)456591Dichlorodihydrofluorescein Diacetate (DCFH)505535DiD - Lipophilic Tracer644665Lipophilic TracerDiD (DiIC18(5)644665DIDS341415Dihydorhodamine 123 (DHR)505535DiI (DiIC18(3)549, 551565Dinitrophenol349DiO (DiOC18(3)484, 487501, 502DiR748780Lipophilic Tr
18、acerDiR (DiIC18(7)750779DM-NERF (high pH)497/510540Ratio Dye, pHDNP349Dopamine340490-520DsRed558583Red fluorescent proteinDTAF494520DY-630-NHS621660Hemicyanelabel for proteins and DNADY-635-NHS634664Hemicyanelabel for proteins and DNAEBFP383447Enhanced Blue Fluorescent ProteinECFP436474Enhanced Cyan
19、 Fluorescent ProteinEGFP488, 498507, 516Enhanced Green Fluorescent ProteinELF 97345530Eosin524545Erythrosin529, 532554, 555Erythrosin ITC529555Ethidium Bromide510, 523595, 605Ethidium homodimer -1 (EthD-1)528617Euchrysin430540EukoLightEuropium (III) chlorideEYFP513, 520527, 532Enhanced Yellow Fluore
20、scent ProteinFast Blue360440FDA494520Feulgen (Pararosaniline)570625FIF (Formaldehyd Induced Fluorescence)405433FITC490, 494520, 525FITC Antibody493517Flazo Orange375-530612Fluo-3480 506, 506520, 527Fluo-4494516Fluorescein (FITC)490, 494520, 525Fluorescein Diacetate494520Fluoro-Emerald495524Fluoro-Go
21、ld (Hydroxystilbamidine)361536Fluor-Ruby555582FluorX494520FM 1-43479598FM 4-46515640Fura Red (high pH)572657Fura Red/Fluo-3Fura-2, high calcium335505Excitation ratio dyeFura-2, low calcium363512Excitation ratio dyeFura-2/BCECFGenacryl Brilliant Red B520590Genacryl Brilliant Yellow 10GF430485Genacryl
22、 Pink 3G470583Genacryl Yellow 5GF430475GeneBlazer (CCF2)GFP (S65T)498516GFP red shifted (rsGFP)498516GFP wild type, non-UV excitation (wtGFP)475509GFP wild type, UV excitation (wtGFP)395509GFPuv385508Gloxalic Acid405460Granular Blue355425Haematoporphyrin530-560580Hoechst 33258345487Hoechst 333423474
23、83Hoechst 34580392440HPTS355465Hydroxycoumarin325-360386-455Hydroxystilbamidine (FluoroGold)361536Hydroxytryptamine400530Indo-1, high calcium330401Emission ratio dyeIndo-1, low calcium346475Emission ratio dyeIndodicarbocyanine (DiD)644665Indotricarbocyanine (DiR)748780Intrawhite Cf360430JC-1514529JO
24、-JO-1530545JO-PRO-1532544LaserPro795812Laurodan355460LDS 751 (DNA)543712LDS 751 (RNA)590607Leucophor PAF370430Leucophor SF380465Leucophor WS395465Lissamine Rhodamine572, 577591, 592Lissamine Rhodamine B577592LIVE/DEAD Kit Animal CellsCalcein/Ethidium homodimer494528517617for more details refertoLOLO
25、-1566580LO-PRO-1568581Lucifer Yellow425, 428528, 536, 540Lyso Tracker Blue373422Lyso Tracker Blue-White466536Lyso Tracker Green504, 534511, 551Lyso Tracker Red490516Lyso Tracker Yellow551576LysoSensor Blue374424LysoSensor Green442505LysoSensor Yellow/Blue384540Mag Green507531Magdala Red (Phloxin B)5
26、24600Mag-Fura Red483/427659/631Ratio Dye, Mg2+Mag-Fura-2369/329369/330508511/491Ratio Dye Ca2+Ratio Dye Mg2+Mag-Fura-5369/330369/332505/500505/482Ratio Dye, Ca2+Ratio Dye, Mg2+Mag-Indo-1349/328349/330480/390480/417Ratio Dye, Ca2+Ratio Dye, Mg2+Magnesium Green506, 507531Magnesium Orange550575Malachit
27、e Green628Marina Blue362459Maxilon Brilliant Flavin 10 GFF450495Maxilon Brilliant Flavin 8 GFF460495Merocyanin555578Methoxycoumarin360410Mitotracker Green FM490516Mitotracker Orange551576Mitotracker Red578599Mitramycin450470Monobromobimane398490Monobromobimane (mBBr-GSH)398500Monochlorobimane380461M
28、PS (Methyl Green Pyronine Stilbene)364395NBD466539NBD Amine450530Nile Red515-555, 559590, 640Nitrobenzoxadidole465510-650Noradrenaline340490-520Nuclear Fast Red289-530580Nuclear Yellow365495Nylosan Brilliant Iavin E8G460510Oregon Green503522Oregon Green 488-X494517Oregon Green503522Oregon Green 4884
29、90, 493514, 520Oregon Green 500497517Oregon Green 514506526Pacific Blue405455Pararosaniline (Feulgen)570625PBFI340/380420Excitation ratio dyePE-Cy5488670PE-Cy7488755, 767PerCP488675PerCP-Cy5.5488710PE-TexasRed Red 613488613Phloxin B (Magdala Red)524600Phorwite AR360430Phorwite BKL370430Phorwite Rev3
30、80430Phorwite RPA375430Phosphine 3R465565PhotoResist365610Phycoerythrin B PE546-565575Phycoerythrin R PE565578PKH26 (Sigma)551567PKH67496520ChromaPMIA341376Pontochrome Blue Black535-553605POPO-1433457POPO-3533574PO-PRO-1435455PO-PRO-3539567Primuline410550Procion Yellow470600Propidium Iodid (PI)(305)
31、, 536, 538617PyMPO412, 415561, 564, 570Pyrene360387Pyronine410540Pyronine B540-590560-650Pyrozal Brilliant Flavin 7GF365495QSY 7560Quinacrine Mustard440510Red 613 PE-TexasRed488613Resorufin571584, 585RH 414532716Rhod-2552576Rhodamine550573Rhodamine 110496, 497520Rhodamine 123507529Rhodamine 5 GLD470
32、565Rhodamine 6G525555Rhodamine B540625Rhodamine B 200523-557595Rhodamine B extra550605Rhodamine BB540580Rhodamine BG540572Rhodamine Green502527Rhodamine Phallicidine558, 542575, 565Rhodamine Phalloidine542565Rhodamine Red570590Rhodamine WT530555Rose Bengal525, 540550-600R-phycocyanineR-phycoerythrin
33、 (PE)565578rsGFP473509red shifted GFP (S65T)S65A471504S65C479507S65L484510S65T488511Sapphire GFP395511SBFI340/380420Excitation ratio dyeSerotonin365520-540Sevron Brilliant Red 2B520595Sevron Brilliant Red 4G500583Sevron Brilliant Red B530590Sevron Orange440530Sevron Yellow L430490sgBFP387450sgBFP (s
34、uper glow BFP)387450Quantums SuperGloGFPAFPssgGFP474488sgGFP (super glow GFP)474509Quantums SuperGloGFPAFPsSITS336436Ion ChannelsSITS (Primuline)395-425450SITS (Stilbene Isothiosulphonic Acid)365460SNAFL calcein506/535535/620Ratio Dye, pHSNAFL-1508/540543/623Ratio Dye, pHSNAFL-2514/543546/630Ratio D
35、ye, pHSNARF calcein552/574590/629Ratio Dye, pHSNARF1576/548635/587Excitation and emission ratio dyeSodium Green506, 507532Na+, K+SpectrumAqua433,/53480/55VysisSpectrumGreen497/30, 509/31538/44, 524/56VysisSpectrumOrange559/38, 560588/48VysisSpectrum Red587, 587/35612, 612/51SPQ (6-methoxy-N-(3-sulfo
36、propyl)quinolinium)344443Stilbene335440Sulphorhodamine B can C520595Sulphorhodamine G Extra470570SYTO 11508, 510527, 530Dye for DNA, RNASYTO 12499, 500522, 519Dye for DNA, RNASYTO 13488, 491509, 514Dye for DNA, RNASYTO 14517, 521549, 547Dye for DNA, RNASYTO 15516, 518546, 555Dye for DNA, RNASYTO 164
37、88, 494518, 525Dye for DNA, RNASYTO 17621634Dye for DNASYTO 18490, 493507, 527Dye for DNA, RNASYTO 20512530Dye for DNASYTO 21494517Dye for DNASYTO 22515535Dye for DNASYTO 23499520Dye for DNASYTO 24490515Dye for DNASYTO 25521556Dye for DNASYTO 40420441Dye for DNASYTO 41430454Dye for DNASYTO 42433460Dye for DNASYTO 43436467Dye for DN